The effects of antivenom administrations on the brain tissue of experimentally envenomed pregnant rats and their pups with Androctonus crassicauda scorpion venom during organogenesis period

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dc.contributor.authorDemirel, Murside Ayse
dc.contributor.authorAlcigir, Mehmet Eray
dc.contributor.authorOzkan, Ozcan
dc.contributor.authorTurkmen, Merve Biskin
dc.date.accessioned2025-01-21T16:45:23Z
dc.date.available2025-01-21T16:45:23Z
dc.date.issued2021
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThis study aims to show the changing effects of Androctonus crassicauda venom and A. crasicauda specific antivenom during pregnancy in brain tissue of dams and their pups. Totally, 12 pregnant-Wistar Albino rats were randomly divided into two groups as venom-antivenom administration (n = 6) and control groups (n = 6). In venom-antivenom administration group (VAV), the sublethal dose of A. crassicauda venom dissolved in 1 mL physiological saline solution was subcutaneously (s.c.) injected into pregnant rats during organogenesis period (between 7 and 13 days of pregnancy). Four hours after each venom injection, 1 mL/s.c. dose of the specific antivenom was administered to rats of VAV group. The rats in control group were given sterile saline solution 1 mL/s. c. In both groups, the fetuses were surgically delivered on the 21st day of pregnancy; dams and pups were sacrificed on postnatal 21 days, and their brain tissues were removed. The brain tissue of dams and their pups were evaluated histopathologically and immunohistochemically. To show the neuronal damages, 8-hydroxy-2deoxyguanosine (8-OHDG) and amyloid beta precursor protein (ABPP) immunoexpressions were scored in cerebrum, cerebellum, pons and medulla oblongata of brain. To show the neuroprotection, reelin and beta-arrestin immunoexpressions were scored again in the same way. In this context, 8-OHDG immunoexpressions were increased in neocortex, hippocampus and nucleus accumbens when compared with that of control group. Amyloid beta precursor protein was negative in both groups. Reelin and beta-arrestin partly increased in fore and mid brain of VAV group as a reaction against neuronal damages when compared with that of control pups. The authors believe that prompt intervention using anti-venom to scorpion envenomation can partly stop neuronal damages. This neuroprotection may be increased to high and serial doses of anti-venom to save neonatal lives.
dc.identifier.doi10.1016/j.toxicon.2021.06.011
dc.identifier.endpage18
dc.identifier.issn0041-0101
dc.identifier.issn1879-3150
dc.identifier.pmid34214578
dc.identifier.scopus2-s2.0-85109200743
dc.identifier.scopusqualityQ2
dc.identifier.startpage13
dc.identifier.urihttps://doi.org/10.1016/j.toxicon.2021.06.011
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25632
dc.identifier.volume200
dc.identifier.wosWOS:000691881600003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofToxicon
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectAndroctonus crassicauda venom; Puppy; Neuronal damages; Neuroprotection; Antivenom
dc.titleThe effects of antivenom administrations on the brain tissue of experimentally envenomed pregnant rats and their pups with Androctonus crassicauda scorpion venom during organogenesis period
dc.typeArticle

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