Preparation and characterization of sulfonyl-hydrazine attached poly(styrene-divinylbenzene) beads for separation of albumin

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dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorŞenkal, Filiz B.
dc.contributor.authorÇelik, Gökçe
dc.contributor.authorArica, M. Yakup
dc.date.accessioned2020-06-25T17:43:58Z
dc.date.available2020-06-25T17:43:58Z
dc.date.issued2007
dc.description.abstractNovel sulfonyl-hydrazine carrying poly(styrene-divinylbenzene), poly(S-DVB)-S-NH2 ion-exchange beads (size between 210 and 425 mu m) were prepared via suspension polymerization and, were first used as an ion-exchange support for adsorption of human serum albumin (HSA) from aqueous solution. The influence of pH, equilibrium time, ionic strength and initial albumin concentration on the adsorption capacity of the poly(SDVB)-S-NH2 ion-exchange beads has been investigated in a batch system and the unmodified poly(S-DVB) beads were used as control system. Maximum HSA adsorption onto poly(S-DVB)-S-NH2 ion-exchange beads was found to be 63.05 mg/g at pH 7.0. The experimental equilibrium data for HSA adsorption onto poly(S-DVB)-S-NH2 beads was well described by the Langmuir isotherm model. Kinetic parameters of this adsorption system were also analyzed for HSA adsorption onto beads and, the first-order rate equations were favorable. Finally, the poly(S-DVB)-S-NH2 beads was used for the purification of HSA from whole human serum and, the purity of the eluted HSA from the beads was determined as 89% by HPLC from single step purification protocol. (c) 2006 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.colsurfa.2006.07.043
dc.identifier.endpage63en_US
dc.identifier.issn0927-7757
dc.identifier.issue1-3en_US
dc.identifier.scopus2-s2.0-33846647582
dc.identifier.scopusqualityQ1
dc.identifier.startpage56en_US
dc.identifier.urihttps://doi.org10.1016/j.colsurfa.2006.07.043
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3967
dc.identifier.volume294en_US
dc.identifier.wosWOS:000244617500008
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids And Surfaces A-Physicochemical And Engineering Aspects
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpoly(styrene-divinylbenzene) beadsen_US
dc.subjecthydrazineen_US
dc.subjectsuspension polymerizationen_US
dc.subjectadsorptionen_US
dc.subjecthuman serum albuminen_US
dc.subjectkinetic parametersen_US
dc.titlePreparation and characterization of sulfonyl-hydrazine attached poly(styrene-divinylbenzene) beads for separation of albuminen_US
dc.typeArticle

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