Factorial Design Optimization of Solid-Phase Microextraction for High-Performance Liquid Chromatography-Ultraviolet Spectrometry Analysis of Polycyclic Aromatic Hydrocarbons in Cigarette Filter Tar
Yükleniyor...
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
A simple and rapid procedure for the determination of polycyclic aromatic hydrocarbons (PAHs) in cigarette filter tar using solid-phase microextraction (SPME) was developed. The analysis was carried out using high-performance liquid chromatography equipped with an ultraviolet detector. The effects of the SPME experimental parameters on the extraction recovery were studied simultaneously using a central composite design (CCD) after a 2(6-2) fractional factorial experimental design. The SPME variables of interest were the extraction temperature, the extraction time, and the stirring speed, as well as the pH and the concentrations of NaCl (%, w/v) and acetonitrile (ACN). The optimal SPME conditions were as follows: an extraction temperature of 65 degrees C, an extraction time of 50min, a stirring speed of 800rpm, 0% NaCl (w/v), 10% ACN in the sample, and a source pH of 8.0. The extraction calibration plots were linear over the range of 0.25-20ng mL(-1) (r(2) > 0.9912) and the limits of detection (LODs) for the 6 PAHs studied were from 0.17-5.02ng cigarette(-1). The relative standard deviation (RSD) ranged from 7.1-13.5% for intra-day variation and from 8.5-18.4% for inter-day variation. The performance of the proposed method was evaluated for extraction and determination PAHs in real samples (various brands of cigarettes). The total amounts of all of the studied PAHs found in the filter tar of the three brands of cigarettes were 320.2, 17.9, and 66.7ng cigarette(-1), respectively.
Açıklama
Anahtar Kelimeler
high-performance liquid chromatography- ultraviolet spectrometry (HPLC-UV), polycyclic aromatic hydrocarbons (PAHs), solid-phase microextraction (SPME), experimental design, cigarette
Kaynak
Polycyclic Aromatic Compounds
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
34
Sayı
2
Künye
closedAccess
