The effect of platelet rich fibrin on growth factor levels in urethral repair

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dc.contributor.authorSoyer, Tutku
dc.contributor.authorAyva, Sebnem
dc.contributor.authorBoybeyi, Ozlem
dc.contributor.authorAslan, Mustafa Kemal
dc.contributor.authorCakmak, Murat
dc.date.accessioned2020-06-25T18:06:56Z
dc.date.available2020-06-25T18:06:56Z
dc.date.issued2013
dc.departmentKırıkkale Üniversitesi
dc.descriptionBayram, Cem/0000-0001-8717-4668; Soyer, Tutku/0000-0003-1505-6042
dc.description.abstractAim: Platelet rich fibrin (PRF) is an autologous source of growth factors and promotes wound healing. An experimental study was performed to evaluate the effect of PRF on growth factor levels in urethral repair. Materials and Methods: Eighteen Wistar albino rats were included in the study. Rats were allocated in three groups (n:6): control (CG), sham (SG), and PRF (PRFG). In SG, a 5 mm vertical incision was performed in the penile urethra and repaired with 10/0 Vicryl (R) under a microscope. In PRFG, during the urethral repair as described in SG, 1 cc of blood was sampled from each rat and centrifuged for 10 minutes at 2400 rpm. PRF obtained from the centrifugation was placed on the repair site during closure. Penile urethras were sampled 24 hours after PRF application in PRFG and after urethral repair in SG. Transforming growth factor beta receptor (TGF-beta-R-CD105), vascular endothelial growth factor (VEGF) and its receptor (VEGF-R), as well as endothelial growth factor receptor (EGFR), were evaluated in subepithelia of the penile skin and urethra. Groups were compared for growth factor levels and growth factor receptor expression with the Kruskal Wallis test. Results: TGF-beta-R levels were significantly decreased in SG when compared to CG (p < 0.05). In PRFG, TGF-beta-R was increased in both subepithelia of penile skin and urethra with respect to SG (p < 0.05). When VEGF levels and its receptor expression were compared between SG and PRFG, VEGF levels were found to be increased in penile skin subepithelium, whereas VEGF-R expressions were decreased in urethral subepithelia in PRFG (p < 0.05). There was no difference between groups for EGFR levels (p > 0.05). Conclusion: Use of PRF after urethral repair increases TGF-beta-R and VEGF expressions in urethral tissue. PRF can be considered as an alternative measure to improve the success of urethral repair. (C) 2013 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipKirikkale University Scientific CouncilKirikkale Universityen_US
dc.description.sponsorshipThis study is presented in 30th Congress of Turkish Pediatric Surgeons, in 2012, Ankara, Turkey and supported by Kirikkale University Scientific Council. Special thanks to Serkan Sahinoglu, MD for his kind contribution to review the English of the manuscript.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.jpedsurg.2013.06.020
dc.identifier.endpage2549en_US
dc.identifier.issn0022-3468
dc.identifier.issn1531-5037
dc.identifier.issue12en_US
dc.identifier.pmid24314201
dc.identifier.scopus2-s2.0-84889571185
dc.identifier.scopusqualityQ1
dc.identifier.startpage2545en_US
dc.identifier.urihttps://doi.org/10.1016/j.jpedsurg.2013.06.020
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5415
dc.identifier.volume48en_US
dc.identifier.wosWOS:000327938200031
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherW B Saunders Co-Elsevier Incen_US
dc.relation.ispartofJournal Of Pediatric Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPlatelet rich fibrinen_US
dc.subjectUrethracutaneous fistulaen_US
dc.subjectGrowth factorsen_US
dc.titleThe effect of platelet rich fibrin on growth factor levels in urethral repairen_US
dc.typeArticle

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