Poly(hydroxyethyl methacrylate-co-methacrylamidoalanine) membranes and their utilization as metal-chelate affinity adsorbents for lysozyme adsorption

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dc.contributor.authorGaripcan, B.
dc.contributor.authorSay, R.
dc.contributor.authorPatir, S.
dc.contributor.authorArica, Y.
dc.contributor.authorDenizli, A.
dc.date.accessioned2020-06-25T17:35:10Z
dc.date.available2020-06-25T17:35:10Z
dc.date.issued2002
dc.departmentKırıkkale Üniversitesi
dc.descriptionGaripcan, Bora/0000-0002-1773-5607;
dc.description.abstractDifferent adsorbents have been reported in the literature for protein purification. The authors have developed a novel and new approach to obtain high protein adsorption capacity utilizing a 2-methacrylamidoalanine-containing membrane. Amino acid ligand 2-methacrylamidoalanine (MAAL) monomer was synthesized using methacryloyl chloride and alanine. Poly(2-hydroxyethylmethacrylate-co-2-methacrylamidoalanine) [p(HEMA-co-MAAL)] membranes were then prepared by UV-initiated photopolymerization of HEMA and MAAL in the presence of an initiator (azobisisobutyronitrile, AIBN). The synthesized MAAL monomer was characterized by NMR. p(HEMA-co-MAAL) membranes were characterized by swelling studies, porosimeter, SEM, FTIR, and elemental analysis. These membranes have macropores in the size range of 5-10 mum. Cu(II) ions (25.9 mmol/m(2)) were chelated on these membranes. p(HEMA-co-MAAL) membranes were used to study the adsorption of lysozyme from aqueous media containing different amounts of lysozyme (0.1-3.0 mg/l) and at different pH values (4.0-8.0). The non-specific adsorption of lysozyme on the pHEMA membranes was negligible (0.9 mug/cm(2)). Incorporation of MAAL increased the lysozyme adsorption significantly up to 2.96 mg/cm(2). The lysozyme adsorption capacity of the Cu(II) incorporated membranes (9.98 mg/cm(2)) was greater than that of the p(HEMA-co-MAAL) membranes. More than 90% of the adsorbed lysozyme was desorbed in I h in the desorption medium containing 1.0 M NaCl and 0.025 M EDTA. The metal-chelate affinity membranes are suitable for repeated use for more than ten cycles without a noticeable loss of capacity.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1163/15685620260178409
dc.identifier.endpage577en_US
dc.identifier.issn0920-5063
dc.identifier.issue5en_US
dc.identifier.pmid12182559
dc.identifier.startpage563en_US
dc.identifier.urihttps://doi.org/10.1163/15685620260178409
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3043
dc.identifier.volume13en_US
dc.identifier.wosWOS:000177648400005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherVsp Bven_US
dc.relation.ispartofJournal Of Biomaterials Science-Polymer Edition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectlysozyme adsorptionen_US
dc.subjectamino acid membranesen_US
dc.subjectpoly(HEMA)en_US
dc.subjectmetal chelatesen_US
dc.titlePoly(hydroxyethyl methacrylate-co-methacrylamidoalanine) membranes and their utilization as metal-chelate affinity adsorbents for lysozyme adsorptionen_US
dc.typeArticle

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