Invertase immobilized on spacer-arm attached poly(hydroxyethyl methacrylate) membrane: Preparation and properties

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dc.contributor.authorArica, MY
dc.contributor.authorSenel, S
dc.contributor.authorAlaeddinoglu, NG
dc.contributor.authorPatir, S
dc.contributor.authorDenizli, A
dc.date.accessioned2020-06-25T17:34:43Z
dc.date.available2020-06-25T17:34:43Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractMicroporous poly(2-hydroxyethyl methacrylate) (pHEMA) membrane was prepared by UV-initiated photopolymerization. The spacer arm (i.e., hexamethylene diamine) was attached covalently and then invertase was immobilized by the condensation reaction of the amino groups of the spacer arm with carboxyl groups of the enzyme in the presence of carbodiimides. The values of the Michael's constant K-m of invertase were significantly larger (ca. 2.5 times) upon immobilization, indicating decreased affinity by the enzyme for its substrate, whereas V-max was smaller for the immobilized invertase. Immobilization improved the pH stability of the enzyme as well as its temperature stability. Thermal stability was found to increase with immobilization and at 70 degrees C the half times for the activity decay were 12 min for the free enzyme and 41 min for the immobilized enzyme. The immobilized enzyme activity was found to be quite stable in repeated experiments. (C) 2000 John Wiley & Sons, Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/(SICI)1097-4628(20000401)75:14<1685
dc.identifier.endpage1692en_US
dc.identifier.issn0021-8995
dc.identifier.issue14en_US
dc.identifier.scopus2-s2.0-0033881215
dc.identifier.scopusqualityQ2
dc.identifier.startpage1685en_US
dc.identifier.urihttps://doi.org/10.1002/(SICI)1097-4628(20000401)75:14<1685
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2861
dc.identifier.volume75en_US
dc.identifier.wosWOS:000085006100001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Incen_US
dc.relation.ispartofJournal Of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpoly(2-hydroxyethyl methacrylate) membraneen_US
dc.subjectspacer armen_US
dc.subjectcovalent bondingen_US
dc.subjectenzyme immobilizationen_US
dc.subjectinvertaseen_US
dc.titleInvertase immobilized on spacer-arm attached poly(hydroxyethyl methacrylate) membrane: Preparation and propertiesen_US
dc.typeArticle

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