Usnic acid causes apoptotic-like death in Leishmania major, L. infantum and L. tropica

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dc.contributor.authorDerici, Mehmet Kursat
dc.contributor.authorCansaran-Duman, Demet
dc.contributor.authorTaylan-Ozkan, Aysegul
dc.date.accessioned2020-06-25T18:29:29Z
dc.date.available2020-06-25T18:29:29Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.descriptionCansaran-Duman, Demet/0000-0003-3759-6664; Ozkan, Aysegul Taylan/0000-0001-8421-3625; Derici, Mehmet Kursat/0000-0002-8260-7492; Cansaran Duman, Demet/0000-0001-5662-2333
dc.description.abstractLeishmaniasis, a deadly parasitic infection, threatens many people worldwide. Since the high cost, toxicity, and resistance are drawbacks of current treatment options, it is necessary to find safer and more effective new antileishmanial drugs. The aim of this study was to determine the antileishmanial activity of usnic acid and its apoptotic mechanism on Leishmania spp. promastigotes. The antileishmanial activity was evaluated by MTT assay and apoptosis-related gene expression was investigated by qRT-PCR. Usnic acid was to be effective against Leishmania major, L. infantum, and L. tropica promastigotes at IC50 =10.76 mu g/ml, 13.34 mu g/ml, and 21.06 mu g/ml, respectively. We also demonstrated a novel mechanism by which usnic acid inhibited proliferation and caused apoptosis; usnic acid upregulated p53, Bax, Casp-3, and Casp-9 gene expression and downregulated the level of Bcl-2 gene expression. Accordingly, the expression level of the P53 gene increased in L. major, L. infantum and L. tropica by 14.4-, 11.8-, and 9.5-fold, respectively, and in contrast, the Bcl-2 gene expression decreased in all three leishmaniasis by 0.8-, 0.8-, and 0.7-fold, respectively. The present study, therefore, revealed that usnic acid played a critical role in the usnic acid-induced apoptotic process in Leishmania species. Usnic acid is easily accessible and an inexpensive agent, and can be considered as an alternative therapeutic agent for Leishmania infections subject to further tests in animal models.en_US
dc.description.sponsorshipHitit UniversityHitit University [TIP19002.16.001]en_US
dc.description.sponsorshipThis study was supported by Hitit University (Grant no: TIP19002.16.001).en_US
dc.identifier.citationDerici, M.K., Cansaran-Duman, D. & Taylan-Özkan, A. Usnic acid causes apoptotic-like death in Leishmania major, L. infantum and L. tropica. 3 Biotech 8, 384 (2018).en_US
dc.identifier.doi10.1007/s13205-018-1409-6
dc.identifier.issn2190-572X
dc.identifier.issn2190-5738
dc.identifier.issue9en_US
dc.identifier.pmid30148034
dc.identifier.scopus2-s2.0-85052228828
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1007/s13205-018-1409-6
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7337
dc.identifier.volume8en_US
dc.identifier.wosWOS:000442748300002
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartof3 Biotech
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectLeishmaniaen_US
dc.subjectLichenen_US
dc.subjectUsnic aciden_US
dc.subjectqRT-PCRen_US
dc.subjectApoptosisen_US
dc.titleUsnic acid causes apoptotic-like death in Leishmania major, L. infantum and L. tropicaen_US
dc.typeArticle

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