Enzymatic removal of phenol and p-chlorophenol in enzyme reactor: Horseradish peroxidase immobilized on magnetic beads

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dc.contributor.authorBayramoglu, Guelay
dc.contributor.authorArica, M. Yakup
dc.date.accessioned2020-06-25T17:44:25Z
dc.date.available2020-06-25T17:44:25Z
dc.date.issued2008
dc.departmentKırıkkale Üniversitesi
dc.description.abstractHorseradish peroxidase was immobilized on the magnetic poly(glycidylmethacrylate-co-methylmethacrylate) (poly(GMA-MMA)), via covalent bonding and used for the treatment of phenolic wastewater in continuous systems. For this purposes, horseradish peroxidase (HRP) was covalently immobilized onto magnetic poly(GMA-MMA) beds using glutaraldehyde (GA) as a coupling agent. The maximum HRP immobilization capacity of the magnetic poly(GMA-XMA)-GA beads was 3.35 mg g(-1). The immobilized HRP retained 79% of the activity of the free HRP used for immobilization. The immobilized RRP was used for the removal of phenol and p-chlorophenol via polymerization of dissolved phenols in the presence of hydrogen peroxide (H2O2). The effect of pH and temperature on the phenol oxidation rate was investigated. The results were compared with the free HRP, which showed that the optimum pH value for the immobilized HRP is similar to that for the free HRR The optimum pH value for free and immobilized HRP was observed at pH 7.0. The optimum temperature for phenols oxidation with immobilized HRP was between 25 and 35 degrees C and the immobilized HRP has more resistance to temperature inactivation than that of the free form. Finally, the immobilized HRP was operated in a magnetically stabilized fluidized bed reactor, and phenols were successfully removed in the enzyme reactor. (C) 2007 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.jhazmat.2007.12.008
dc.identifier.endpage155en_US
dc.identifier.issn0304-3894
dc.identifier.issn1873-3336
dc.identifier.issue1-3en_US
dc.identifier.pmid18207637
dc.identifier.scopus2-s2.0-44649117556
dc.identifier.scopusqualityQ1
dc.identifier.startpage148en_US
dc.identifier.urihttps://doi.org/10.1016/j.jhazmat.2007.12.008
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4103
dc.identifier.volume156en_US
dc.identifier.wosWOS:000257722000021
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal Of Hazardous Materials
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjecthorseradish peroxidaseen_US
dc.subjectimmobilizationen_US
dc.subjectphenol removalen_US
dc.subjectwastewater treatmenten_US
dc.subjectenzyme reactoren_US
dc.titleEnzymatic removal of phenol and p-chlorophenol in enzyme reactor: Horseradish peroxidase immobilized on magnetic beadsen_US
dc.typeArticle

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