Adsorption of IgG on spacer-arm and L-arginine ligand attached poly(GMA/MMA/EGDMA) beads

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dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorŞenel, Ayşegül U.
dc.contributor.authorArica, M. Yakup
dc.date.accessioned2020-06-25T17:43:51Z
dc.date.available2020-06-25T17:43:51Z
dc.date.issued2007
dc.description.abstractThis work presents data on human imrnunoglobulin G (HlgG) adsorption onto L-arginine ligand attached poly(GMA/MMA/EGDMA)-based affinity beads which were synthesized from methyl methacrylate (NUVIA) and glycidiyl methacrylate (GMA) in the presence of a crosslinker (i.e., ethylene glycol dimethacrylate; EGDMA) by suspension polymerization. The epoxy groups of the poly(GMA/NMA/ EGDMA) beads were converted into amino groups after reaction with ammonia or 1,6-diaminohexane (i.e., spacer-arm). With L-arginine as a ligand, it was covalently immobilized on the an-dnated (poly(GMA/MMA/EGDMA)AA) and/or the spacer-arm attached (poly(GMA/NMA/ EGDM.A)-SA) beads, using glutaric dialdehyde as a coupling agent. Both affinity poly(GMA/MMA/EGDMA)-based beads were used in HlgG adsorption/desorption studies under defined pH, ionic strength, or temperature conditions in a batch reactor, using acid-treated poly(GMA/MMA/EGDMA) beads as a control system. The poly(GMA/MMA/EGDMA)-SA affinity beads resulted in an increase in the adsorption capacity to HlgG compared with the aminated counterpart (i.e., poly(GMA/MMA/EGDMA)-AA). The maximum adsorption capacities of the poly(GMA/MMA/EGDMA)-AA and poly(GMA/MNA/EGDMA)-SA affinity beads were found to be 112.36 and 142 mg g(-1), and the affinity constants (K-d), evaluated by the Langmuir model, were 2.48 x 10(-7) and 6.98 x 10(-7) M, respectively. Adsorption capacities of the poly(GMA/MMA/EGDMA)-AA and poly(GMA/MMA/EGDMA)-SA were decreased with HIgG by increasing the ionic strength adjusted with NaCl. Adsorption kinetic of HIgG onto both affinity adsorbents was analyzed with first- and second-order kinetic equations. The first-order equation fitted well with the experimental data. (c) 2007 Wiley Periodicals, Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/app.25737
dc.identifier.endpage679en_US
dc.identifier.issn0021-8995
dc.identifier.issn1097-4628
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-33947272562
dc.identifier.scopusqualityQ2
dc.identifier.startpage672en_US
dc.identifier.urihttps://doi.org10.1002/app.25737
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3922
dc.identifier.volume104en_US
dc.identifier.wosWOS:000244375100091
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofJournal Of Applied Polymer Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectbeadsen_US
dc.subjectpolymerizationen_US
dc.subjectaffinity chromatographyen_US
dc.subjectamino aciden_US
dc.subjectIgGen_US
dc.subjectthermodynamicsen_US
dc.titleAdsorption of IgG on spacer-arm and L-arginine ligand attached poly(GMA/MMA/EGDMA) beadsen_US
dc.typeArticle

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