Separation and purification of lysozyme by Reactive Green 19 immobilised membrane affinity chromatography

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dc.contributor.authorYılmaz, Meltem
dc.contributor.authorBayramoğlu, Gülay
dc.contributor.authorArıca, M. Yakup
dc.date.accessioned2020-06-25T17:40:51Z
dc.date.available2020-06-25T17:40:51Z
dc.date.issued2005
dc.departmentKırıkkale Üniversitesi
dc.description.abstractA poly(hydroxyethylmethacrylate)/chitosan (pHEMA-chitosan) composite membrane was prepared by UV-initiated photopolyrnerisation. A dye ligand (i.e. Reactive Green 19; RG 19) was immobilised onto the membrane. The water content of the dye ligand-immobilised membrane was 50 +/- 2% and the amount of immobilised Reactive Green 19 on the membrane was 0.865 mumol/mi. The rates of adsorption of lysozyme on plain composite and dye ligand-immobilised composite membranes were measured in a stirred cell. The adsorption capacity of the composite membrane was determined by changing pH and the concentration of lysozyme in the adsorption medium. Separation and purification of lysozyme from aqueous solution and egg white were investigated, respectively. The lysozyme adsorption capacity of the RG 19 immobilised membrane was 60.8 mg/ml. The adsorption capacity of the plain composite membrane was 7.2 mg/ml. Separation and purification were monitored by determining the lysozyme activity using Micrococcus lysodeikticus as substrate. The lysozyme was purified 25.4-fold in a single step with a recovery 82%, as determined by HPLC. The membranes were stable when subjected to sanitization with sodium hydroxide after repeated adsorption-elution cycles. (C) 2004 Elsevier Ltd. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.foodchem.2004.01.072
dc.identifier.endpage18en_US
dc.identifier.issn0308-8146
dc.identifier.issn1873-7072
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-3543148365
dc.identifier.scopusqualityQ1
dc.identifier.startpage11en_US
dc.identifier.urihttps://doi.org/10.1016/j.foodchem.2004.01.072
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3566
dc.identifier.volume89en_US
dc.identifier.wosWOS:000223925200002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofFood Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectaffinity chromatographyen_US
dc.subjectcomposite membraneen_US
dc.subjectdye-liganden_US
dc.subjectlysozymeen_US
dc.subjectadsorptionen_US
dc.subjectpurificationen_US
dc.titleSeparation and purification of lysozyme by Reactive Green 19 immobilised membrane affinity chromatographyen_US
dc.typeArticle

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