Could radial extracorporeal shock wave therapy have an effect on wound healing in clinical practice by creating genotoxic damage? An in vitro study in mouse fibroblasts

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dc.contributor.authorŞimşek, Ekin Kaya
dc.contributor.authorHaberal, Bahtiyar
dc.contributor.authorKasap, Yeşim Korkmaz
dc.contributor.authorYurtcu, Erkan
dc.date.accessioned2025-01-21T16:27:12Z
dc.date.available2025-01-21T16:27:12Z
dc.date.issued2021
dc.departmentKırıkkale Üniversitesi
dc.description.abstractObjectives: This study aims to evaluate wound healing effects of in vitro radial extracorporeal shock wave (rESW) application on mouse fibroblasts and whether the cytotoxic effect of extracorporeal shock wave (ESW) was due to a possible genotoxic effect. Patients and methods: After creating an in vitro wound healing model in L929 mouse fibroblast culture, fibroblasts were stimulated with a frequency of 3 Hz, and 100, 250, 500, 1,000 and 1,500 pulses shock waves were applied. Energy flux densities ranging from 0.01 to 0.23 mJ/mm2 (14.3 MPa) at a constant pressure level of 0.5 and 1 bar were applied. Wound healing, cell viability, and genotoxicity were evaluated at 24 and 48 h. Results: All shot numbers for both pressures significantly reduced cell viability (p<0.05). For both 0.5 and 1 bar pressures, in both intervals, the rate of wound healing decreased, regardless of the number of shots (p<0.05). In vitro genotoxic damage was detected at both 0.5 and 1 bar pressures, in both time intervals, regardless of the number of shots. The genotoxic damage increased from 24 to 48 h. Conclusion: The study results suggest that, when ESWT is applied in this in vitro experimental setup, cell viability decreases and wound healing is delayed under all conditions. Furthermore, genotoxic damage can be prevented by using shots below 1,000 pulses. Therefore, while investigating the therapeutic effect of ESW therapy in vitro, the upper limit for the number of shots should be 1,000 pulses. © 2021 the Turkish Joint Diseases Foundation. All Rights Reserved.
dc.description.sponsorshipBaşkent University
dc.identifier.doi10.52312/JDRS.2021.315
dc.identifier.endpage667
dc.identifier.issn2687-4792
dc.identifier.issue3
dc.identifier.pmid34842098
dc.identifier.scopus2-s2.0-85121214297
dc.identifier.scopusqualityQ2
dc.identifier.startpage658
dc.identifier.trdizinid474698
dc.identifier.urihttps://doi.org/10.52312/JDRS.2021.315
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/474698
dc.identifier.urihttps://hdl.handle.net/20.500.12587/23293
dc.identifier.volume32
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTurkish Joint Diseases Foundation
dc.relation.ispartofJoint Diseases and Related Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectCell viability; extracorporeal shock wave therapy; genotoxicity; in vitro; wound healing
dc.titleCould radial extracorporeal shock wave therapy have an effect on wound healing in clinical practice by creating genotoxic damage? An in vitro study in mouse fibroblasts
dc.typeArticle

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