Effects Of Long-Term Storage On Some Spermatological Parameters In Cryopreserved Bull Semen

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dc.contributor.authorAkyol, Numan
dc.contributor.authorVarisli, Omer
dc.contributor.authorKizil, Sedat Hamdi
dc.date.accessioned2020-06-25T18:29:21Z
dc.date.available2020-06-25T18:29:21Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.description.abstractBACKGROUND: The effect of the long-term storage in liquid nitrogen on semen quality has not be reported. OBJECTIVE: The study measured the spermatological parameters of bull sperm after the long-term and short-term storage. MATERIALS AND METHODS: Vintage semen (obtained from 5 Brown Swiss bulls and frozen 30 years ago) and newly frozen semen collected from 5 bulls of the same breed and prepared at the International Center for Livestock Research and Training were used. For each bull, 10 straws (0.25 ml) were thawed and pooled. Sperm samples were analyzed by flow cytometry, computer assisted sperm analysis, and total oxidant-antioxidant levels were also tested. RESULTS: The ratios of necrotic (P < 0.001) and apoptotic (P = 0.006) spermatozoa, and the ratios of total antioxidant status (TAS) and total oxidant status (TOS) were significantly higher (P < 0.001) in long-term frozen spermatozoa. However, the early necrotic ratios (P < 0.001), velocity average pathway (VAP) (P = 0.008) and velocity curvi linear (VCL) (P = 0.01) values of long-term frozen semen were lower compared with short-term frozen semen. While necrotic and apoptotic spermatozoa ratios and total oxidant level were higher, VAPNCL ratios were lower in long-term frozen sperms compared to short-term frozen semen. CONCLUSION: Long-term storage of sperm may adversely affect the spermatological and oxidative parameters of Brown Swiss bull spermatozoa.en_US
dc.description.sponsorshipKirikkale University Scientific Research Projects Coordination UnitKirikkale University [2016/037]en_US
dc.description.sponsorshipThe research is supported by the Kirikkale University Scientific Research Projects Coordination Unit (Project #2016/037).en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.endpage358en_US
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue6en_US
dc.identifier.pmid30963151
dc.identifier.scopus2-s2.0-85061133794
dc.identifier.scopusqualityQ3
dc.identifier.startpage354en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7253
dc.identifier.volume39en_US
dc.identifier.wosWOS:000455571800002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherCryo Lettersen_US
dc.relation.ispartofCryoletters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBull semenen_US
dc.subjectlong-term storageen_US
dc.subjectcryopreservationen_US
dc.subjectBrown Swissen_US
dc.titleEffects Of Long-Term Storage On Some Spermatological Parameters In Cryopreserved Bull Semenen_US
dc.typeArticle

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