Performance of different metal-dye chelated affinity adsorbents of poly(2-hydroxyethyl methacrylate) in lysozyme separation

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dc.contributor.authorArica, M. Yakup
dc.contributor.authorDenizli, A.
dc.date.accessioned2020-06-25T17:34:45Z
dc.date.available2020-06-25T17:34:45Z
dc.date.issued2000
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe triazine dye Cibacron Blue F3GA was covalently immobilized as an affinity ligand onto microporous poly(2-hydroxyethyl methacrylate) (pHEMA) membranes. Three different metal ions [i.e.. Fe(III), Zn(II), or Cu(II)] were then chelated with the immobilized Cibacron Blue F3GA molecules. Lysozyme adsorption onto these affinity adsorbents from aqueous solutions containing different amounts of lysozyme at different pH was investigated in a batch system. Lysozyme adsorption capacity of all of the metal-dye-immobilized membranes was greater than that of the dye-immobilized membranes. The nonspecific adsorption of the protein on the pHEMA membranes was negligible. The adsorption phenomena appeared to follow a typical Lang muir isotherm. The maximum capacity (q(m)) of the Fe(III)-Zn(II),-or Cu(II)-dye chelated membranes for lysozyme adsorption (384, 326, and 306 mug/cm(2)) was greater than that of the dye-immobilized membrane (224 mug lysozyme/cm(2)), respectively. The dissociation constant (k(d)) values were found to be 2.51 x 10(-7) M with dye-immobilized membrane, and 2.32 X 10(-7), 2.38 X 10(-7), and 2.40 x 10(-7) M with the Fe(III)-Zn(II),-and Cu(II)-dye-chelated membranes, respectively. More than 95% of the adsorbed lysozyme was desorbed in 60 min in the desorption medium containing 0.5 M KSCN at pH 8.0.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1081/SS-100102100
dc.identifier.endpage2257en_US
dc.identifier.issn0149-6395
dc.identifier.issue14en_US
dc.identifier.scopus2-s2.0-0033664137
dc.identifier.scopusqualityQ2
dc.identifier.startpage2243en_US
dc.identifier.urihttps://doi.org/10.1081/SS-100102100
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2876
dc.identifier.volume35en_US
dc.identifier.wosWOS:000168274700005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherMarcel Dekker Incen_US
dc.relation.ispartofSeparation Science And Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectlysozymeen_US
dc.subjectmicroporous membraneen_US
dc.subjectCibacron Blue F3GAen_US
dc.subjectaffinity adsorptionen_US
dc.subjectmetal chelateen_US
dc.titlePerformance of different metal-dye chelated affinity adsorbents of poly(2-hydroxyethyl methacrylate) in lysozyme separationen_US
dc.typeArticle

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