Comparison of adsorption performances of metal-chelated polyamide hollow fibre membranes in lysozyme separation

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dc.contributor.authorŞenel, S.
dc.contributor.authorKassab, A.
dc.contributor.authorArıca, Y.
dc.contributor.authorSay, R.
dc.contributor.authorDenizli, A.
dc.date.accessioned2020-06-25T17:35:05Z
dc.date.available2020-06-25T17:35:05Z
dc.date.issued2002
dc.departmentKırıkkale Üniversitesi
dc.description.abstractCommercially available microporous polyamide hollow fibres are modified by acid hydrolysis to activate the reactive groups and subsequently binding of the ligand, i.e. Cibacron Blue F3GA. Then the Cibacron Blue F3GA-derived hollow fibres were loaded with different metal ions (i.e. Zn(II), Cu(II), Ni(II)) to form the metal chelate. The internal polymer matrix was characterised by scanning electron microscopy. The effects of pH, initial concentration of lysozyme, metal type and temperature on the adsorption of lysozyme to the metal-chelated hollow fibres were examined in a batch reactor. The non-specific adsorption of lysozyme onto the polyamide hollow fibres was 1.8 mg/g. Cibacron Blue F3GA immobilisation increased the lysozyme adsorption up to 62.3 mg/g. Metal-chelated hollow fibres showed a significant increase of the adsorption efficiency. Lysozyme adsorption capacities of Zn(II), Cu(II) and Ni(Il)-chelated hollow fibres were different. The maximum capacities of Zn(II), Cu(II) or Ni(Il)-chelated hollow fibres were 144.2, 75.2 and 68.6 mg/g, respectively. Significant amount of the adsorbed lysozyme (up to 97%) was eluted in 1 h in the elution medium containing 1.0 M NaSCN at pH 8.0 and 25 mM EDTA at pH 4.9. Repeated adsorption-desorption process showed that this novel metal-chelated polyamide hollow fibres are suitable for lysozyme adsorption. (C) 2002 Published by Elsevier Science B.V.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0927-7765(01)00278-8
dc.identifier.endpage275en_US
dc.identifier.issn0927-7765
dc.identifier.issue3-4en_US
dc.identifier.scopus2-s2.0-0036179182
dc.identifier.scopusqualityQ1
dc.identifier.startpage265en_US
dc.identifier.urihttps://doi.org/10.1016/S0927-7765(01)00278-8
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3014
dc.identifier.volume24en_US
dc.identifier.wosWOS:000174555700010
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids And Surfaces B-Biointerfaces
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectpolyamide hollow fibresen_US
dc.subjectdye-chelate chromatographyen_US
dc.subjectlysozymeen_US
dc.subjectCibacron Blue F3GAen_US
dc.titleComparison of adsorption performances of metal-chelated polyamide hollow fibre membranes in lysozyme separationen_US
dc.typeArticle

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