Gene expression profiles of vitrified in vitro- and in vivo-derived bovine blastocysts

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dc.contributor.authorAksu, Digdem Aktoprakligil
dc.contributor.authorAgca, Cansu
dc.contributor.authorAksu, Soner
dc.contributor.authorBagis, Haydar
dc.contributor.authorAkkoc, Tolga
dc.contributor.authorCaputcu, Arzu Tas
dc.contributor.authorAgca, Yuksel
dc.date.accessioned2020-06-25T17:52:42Z
dc.date.available2020-06-25T17:52:42Z
dc.date.issued2012
dc.departmentKırıkkale Üniversitesi
dc.descriptionKARASAHIN, Tahir/0000-0003-2358-0389; BAGIS/0000-0002-1140-8058; Taskin, Ali Cihan/0000-0003-3196-821X; NUR, ZEKARIYA/0000-0002-1438-221X
dc.description.abstractVitrification is becoming a preferred method for pre-implantation embryo cryopreservation. The objective of this study was to determine the differentially expressed genes of in vivo- and in vitro-produced bovine embryos after vitrification. In vitro- (IVF) and in vivo-derived (IVV) bovine blastocysts were identified as follows: in vitro-produced fresh (IVF-F), in vitro-produced vitrified (IVF-V), in vivo-derived fresh (IVV-F), in vivo-derived vitrified (IVV-V). The microarray results showed that 53 genes were differentially regulated between IVF and IVV, and 121 genes were differentially regulated between fresh and vitrified blastocysts (P?<?0.05). There were 6, 268, 962, and 17 differentially regulated genes between IVF-F x IVV-F, IVF-V x IVV-V, IVF-F x IVF-V, and IVV-F x IVV-V, respectively (P?<?0.05). While gene expression was significantly different between fresh and vitrified IVF blastocysts (P?<?0.05), it was similar between fresh and vitrified IVV blastocysts. Significantly up-regulated KEGG pathways included ribosome, oxidative phosphorylation, spliceosome, and oocyte meiosis in the fresh IVF blastocyst samples, while sphingolipid and purine metabolisms were up-regulated in the vitrified IVF blastocyst. The results showed that in vitro bovine blastocyst production protocols used in this study caused no major gene expression differences compared to those of in vivo-produced blastocysts. After vitrification, however, in vitro-produced blastocysts showed major gene expression differences compared to in vivo blastocysts. This study suggests that in vitro-produced embryos are of comparable quality to their in vivo counterparts. Vitrification of in vitro blastocysts, on the other hand, causes significant up-regulation of genes that are involved in stress responses. Mol. Reprod. Dev. 79: 613625, 2012. (C) 2012 Wiley Periodicals, Inc.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [KAMAG-107G027]en_US
dc.description.sponsorshipGrant sponsor: The Scientific and Technological Research Council of Turkey (TUBITAK); Grant number: KAMAG-107G027en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/mrd.22068
dc.identifier.endpage625en_US
dc.identifier.issn1040-452X
dc.identifier.issn1098-2795
dc.identifier.issue9en_US
dc.identifier.pmid22778065
dc.identifier.scopus2-s2.0-84866317684
dc.identifier.scopusqualityQ2
dc.identifier.startpage613en_US
dc.identifier.urihttps://doi.org/10.1002/mrd.22068
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5211
dc.identifier.volume79en_US
dc.identifier.wosWOS:000308886100004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofMolecular Reproduction And Development
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleGene expression profiles of vitrified in vitro- and in vivo-derived bovine blastocystsen_US
dc.typeArticle

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