The Effects of Beta-blockers on Endothelial Nitric Oxide Synthase Immunoreactivity in the Rat Corpus Cavernosum

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dc.contributor.authorDogru, Mehmet Tolga
dc.contributor.authorAydos, Tolga Resat
dc.contributor.authorAktuna, Zuhal
dc.contributor.authorKorkusuz, Petek
dc.contributor.authorZeybek, Dilara
dc.contributor.authorGorgu, Nart
dc.contributor.authorBasar, Mehmet Murad
dc.date.accessioned2020-06-25T17:51:18Z
dc.date.available2020-06-25T17:51:18Z
dc.date.issued2010
dc.departmentKırıkkale Üniversitesi
dc.descriptionAydos, Tolga Resat/0000-0002-1832-9336; KORKUSUZ, PETEK/0000-0002-7553-3915; Zeybek, Dilara/0000-0002-6161-5661
dc.description.abstractOBJECTIVES To explain the mechanism of the effects of beta-blockers on endothelial dysfunction and release of nitric oxide from the endothelium. METHODS A total of 72 Sprague-Dawley rats were divided into 9 different groups as follows: group 1: control (n = 10), group 2: metoprolol (Beloc) 100 mg/kg/d (n = 7), group 3: carvedilol (Dilatrend) 50 mg/kg/d (n = 7), group 4: nebivolol (Vasoxen) 10 mg/kg/d (n = 6), group 5: estrogen receptor (ER) antagonist ICI 182.780 (Fluvestrant) 50 mu g/g (n = 10), group 6: nebivolol +ER antagonist (n = 8), group 7: androgen receptor (AR) antagonist (flutamide) 20 mg/kg (n = 7), group 8: nebivolol + AR antagonist (n = 7), and group 9: DMSO (solvent for ER antagonist) (n = 10). All beta-blockers were applied with gastric gavage after dilution with 5 mL of serum physiological; ER and AR were both applied intraperitoneally (i.p.) for 14 days. In the isolated rat cavernous tissues, endothelial nitric oxide synthase (eNOS) and ER and AR immunoreactivity were analyzed quantitatively. One-way analysis of variance and Tukey test were used for statistical analysis. RESULTS Although increased eNOS immunoreactivity was observed with nebivolol and nebivolol-flutamide in endothelial cells laying cavernous tissue, a lower score was observed after ICI-182.780 application, when compared with control cases. AR immunoreactivity in cavernosal endothelium was clearly higher with nebivolol. Higher H score and ER immunoreactivity were observed in the cavernous endothelium and smooth muscles in the nebivolol, carvedilol, and metoprolol groups when compared with control cases. CONCLUSIONS We showed that eNOS activity was increased in the nebivolol and nebivolol-flutamide groups, whereas it was decreased in the ICI 182.780 group. We believe that an ER-dependent mechanism triggered by nebivolol played a role in nitric oxide formation. UROLOGY 75: 589-597, 2010. (C) 2010 Elsevier Inc.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.urology.2009.09.025
dc.identifier.endpage597en_US
dc.identifier.issn0090-4295
dc.identifier.issn1527-9995
dc.identifier.issue3en_US
dc.identifier.pmid19962728
dc.identifier.scopus2-s2.0-77649183489
dc.identifier.scopusqualityQ2
dc.identifier.startpage589en_US
dc.identifier.urihttps://doi.org/10.1016/j.urology.2009.09.025
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4788
dc.identifier.volume75en_US
dc.identifier.wosWOS:000275814500036
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Incen_US
dc.relation.ispartofUrology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.titleThe Effects of Beta-blockers on Endothelial Nitric Oxide Synthase Immunoreactivity in the Rat Corpus Cavernosumen_US
dc.typeArticle

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