Changes in the size distribution of steroidogenic and non-steroidogenic bovine luteal cells during the oestrus cycle

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dc.contributor.authorYigit, A
dc.contributor.authorArikan, S
dc.date.accessioned2020-06-25T17:34:55Z
dc.date.available2020-06-25T17:34:55Z
dc.date.issued2001
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe aim of this study was to investigate the size distribution of steroidogenic and non-steroidogenic luteal cells throughout the oestrus cycle. Twelve corpora lutea collected from different stages of the oestrus cycle were used. Corpora lutea collected from nonpregnant cows were dissociated into single-cell suspensions by enzyme treatments. Cells were stained for 3 beta -hydroxysteroid dehydrogenase (3 beta -HSD) activity, a marker for steroidogenic cells. The sizes of 3 beta -HSD positive (steroidogenic) and negative cells (nonsteroidogenic) were measured with an ocular micrometer. Very small cells (<10 <mu>m) stained negative for 3 beta -HSD activity were presumed to be primarily endothelial cells, fibroblast and blood cells. The other cells were presumed to be luteal cells covering a wide spectrum of size ranging from 10 to 40 mum in diameter. 3 beta -HSD positive cells smaller than 10 mum were not observed, and cells larger than 25 mum were rare. The cells obtained from early and late stages of the luteal phase contained more non-steroidogenic cells than the cells obtained from the mid-luteal phase. However, the cells derived from early luteal stage contained more small cells (10-22 mum) than the cells obtained from the rest of the cycle. There were significant differences (p < 0.001) in both 3<beta>-HSD positive and 3 beta -HSD negative cell sizes during the oestrus cycle. This study indicates that the size distributions of both steroidogenic and non-steroidogenic luteal cells change as the age of the corpus luteum increases.en_US
dc.identifier.citationYiğit, A. A., Arıkan, Ş. (2001). İneklerde östrus siklusu süresince gelişen steroidojenik ve non-steroidojenik luteal hücrelerin büyüklük dağılımlarında meydana gelen değişiklikler. Turkish Journal of Veterinary and Animal Sciences, 25(4), 545 - 550.en_US
dc.identifier.endpage550en_US
dc.identifier.issn1300-0128
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-0035606153
dc.identifier.scopusqualityQ3
dc.identifier.startpage545en_US
dc.identifier.trdizinidtrdizinikyoktur
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2950
dc.identifier.volume25en_US
dc.identifier.wosWOS:000168884800018
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isotr
dc.publisherScientific Technical Research Council Turkeyen_US
dc.relation.ispartofTurkish Journal Of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectbovineen_US
dc.subject3 beta-HSDen_US
dc.subjectluteal cellsen_US
dc.subjectoestrus cycleen_US
dc.titleChanges in the size distribution of steroidogenic and non-steroidogenic bovine luteal cells during the oestrus cycleen_US
dc.typeArticle

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