Assessment of the effects of levosimendan and thymoquinone on lung injury after myocardial ischemia reperfusion in rats

Basit Öğe Kaydı
dc.contributor.authorSezen, Saban Cem
dc.contributor.authorKucuk, Aysegul
dc.contributor.authorOzer, Abdullah
dc.contributor.authorKilic, Yigit
dc.contributor.authorMardin, Baris
dc.contributor.authorAlkan, Metin
dc.contributor.authorTosun, Murat
dc.date.accessioned2020-06-25T18:30:04Z
dc.date.available2020-06-25T18:30:04Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.descriptionArslan, Mustafa/0000-0003-4882-5063
dc.description.abstractAim: The aim of this study was to investigate the effects of levosimendan and thymoquinone (TQ) on lung injury after myocardial ischemia/reperfusion (I/R). Materials and methods: Twenty-four Wistar albino rats were included in the study. The animals were randomly assigned to 1 of 4 experimental groups. In Group C (control group), left anterior descending artery was not occluded or reperfused. Myocardial I/R was induced by ligation of the left anterior descending artery for 30 min, followed by 2 h of reperfusion in the I/R, I/R-levosimendan (24 mu g/kg) (IRL) group, and I/R-thymoquinone (0.2 mL/kg) (IRTQ) group. Tissue samples taken from the lungs of rats were histochemically stained with H&E and immunohistochemically stained with p53, Bcl 2, Bax, and caspase 3 primer antibodies. Results: Increased expression of p53 and Bax was observed (4+), especially in the I/R group. In IRTQ and IRL groups, expression was also observed at various locations (2+, 3+). H&E staining revealed that that the lungs were severely damaged and the walls of the alveoli were too thick, the number of areas examined was increased during the evaluation. Caspase 3 expression was observed to be at an (1+, 2+) intensity that was usually weak and diffuse in multiple areas. Bcl 2 was not found to be expressed in any of the tissues. H&E staining revealed that that the lungs were severely damaged in the I/R group, with the walls of the channels and alveoli thickened and edematous, and also an intense inflammatory cell migration was observed. Immunohistochemical staining was more prominent in inflammatory areas and structures around the terminal bronchioles. Conclusion: The findings in our study have shown that administration of levosimendan and TQ during I/R increases expression of caspase 3, p53, and Bax in lung tissue and has a protective effect on lung as distant organ. We suggest that findings of this study be elucidated with further large-scale clinical studies.en_US
dc.identifier.citationSezen SC, Kucuk A, Özer A, Kılıç Y, Mardin B, Alkan M, Erkent FD, Arslan M, Ünal Y, Oktar GL, Tosun M. Assessment of the effects of levosimendan and thymoquinone on lung injury after myocardial ischemia reperfusion in rats. Drug Des Devel Ther. 2018;12:1347-1352.en_US
dc.identifier.doi10.2147/DDDT.S160092
dc.identifier.endpage1352en_US
dc.identifier.issn1177-8881
dc.identifier.pmid29861626
dc.identifier.scopus2-s2.0-85047782860
dc.identifier.scopusqualityQ1
dc.identifier.startpage1347en_US
dc.identifier.urihttps://doi.org/10.2147/DDDT.S160092
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7548
dc.identifier.volume12en_US
dc.identifier.wosWOS:000433205000001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherDove Medical Press Ltden_US
dc.relation.ispartofDrug Design Development And Therapy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectischemia reperfusionen_US
dc.subjectlevosimendanen_US
dc.subjectthymoquinoneen_US
dc.subjectlungen_US
dc.subjectcaspase 3en_US
dc.subjectp53en_US
dc.titleAssessment of the effects of levosimendan and thymoquinone on lung injury after myocardial ischemia reperfusion in ratsen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Assessment of the effects of levosimendan and thymoquinone on lung injury after myocardial ischemia reperfusion in rats.pdf
Boyut:
3.81 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu