Preparation and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHX) based nanoparticles for targeted cancer therapy

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dc.contributor.authorKilicay, Ebru
dc.contributor.authorDemirbilek, Murat
dc.contributor.authorTurk, Mustafa
dc.contributor.authorGuven, Eylem
dc.contributor.authorHazer, Baki
dc.contributor.authorDenkbas, Emir Baki
dc.date.accessioned2020-06-25T17:51:36Z
dc.date.available2020-06-25T17:51:36Z
dc.date.issued2011
dc.departmentKırıkkale Üniversitesi
dc.descriptionGUVEN, EYLEM/0000-0001-9039-1698
dc.description.abstractTargeted drug delivery systems are one of the most promising alternatives for the cancer therapy. Rapid developments on nanomedicine facilitated the creation of novel nanotherapeutics by using different nanomaterials. Especially polymer based nanoparticles are convenient for this purpose. In this study; a natural polymer (poly(3-hydroxybutyrate-co-3-hydroxyhexanoate), PHBHHX) was used as a base matrix for the production of a novel nanotherapeutic including antineoplastic agent, Etoposide and attached folic acid as a ligand on the nanoparticles. Modified solvent evaporation technique was used for the production of PHBHHX nanoparticles and the average size of the obtained PHBHHX nanoparticles were observed in the range of 180 nm and 1.5 mu m by the change in experimental conditions (i.e., homogenization rate, surfactant concentration and polymer/solvent ratio). By the increase in homogenization rate and surfactant concentration, size of the nanoparticles was decreased, while the size was increased by the increase in polymer/solvent ratio. Drug loading ratio was also found to be highly affected by polymer/drug ratio. Surface charge of the prepared nanoparticles was also investigated by zeta potential measurements. In the cytotoxicity tests; Etoposide loaded and folic acid attached PHBHHX nanoparticles were observed as more effective on HeLa cells than Etoposide loaded PHBHHX nanoparticles without attached folic acid. The cytotoxicity of folic acid conjugated PHBHHX nanoparticles to cancer cells was found to be much higher than that of normal fibroblast cells, demonstrating that the folate conjugated nanoparticles has the ability to selectively target to cancer cells. In addition, apoptotic/necrotic activities were evaluated for all formulations of the PHBHHX nanoparticles and parallel results with cytotoxicity tests were obtained. These studies demonstrate that the folic acid attached and Etoposide loaded PHBHHX nanoparticles seem as promising for the targeted cancer therapy. (C) 2011 Elsevier B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/j.ejps.2011.08.013
dc.identifier.endpage320en_US
dc.identifier.issn0928-0987
dc.identifier.issn1879-0720
dc.identifier.issue3en_US
dc.identifier.pmid21884788
dc.identifier.scopus2-s2.0-80053902022
dc.identifier.scopusqualityQ1
dc.identifier.startpage310en_US
dc.identifier.urihttps://doi.org/10.1016/j.ejps.2011.08.013
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4923
dc.identifier.volume44en_US
dc.identifier.wosWOS:000296930000016
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofEuropean Journal Of Pharmaceutical Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTargeted cancer therapyen_US
dc.subjectPHBHHX nanoparticlesen_US
dc.subjectEtoposideen_US
dc.subjectFolic aciden_US
dc.subjectNanoparticle-cell interactionsen_US
dc.titlePreparation and characterization of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHX) based nanoparticles for targeted cancer therapyen_US
dc.typeArticle

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