Immobilization of a- amylase on reactive modified fiber and its application for continuous starch hydrolysis in a packed bed bioreactor
Yükleniyor...
Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, the enzyme -amylase was immobilized on reactive modified poly (ethylene terephthalate) fiber. The activities of free -amylase and immobilized -amylase were compared in a batch starch hydrolysis system. Optimum pH and Michaelis-Menten constants were determined for both free -amylase and immobilized -amylase. The immobilization shifted the optimum pH to a higher level. The Michaelis-Menten constant values (K-m) of the immobilized -amylase and free -amylase were obtained as 11.94 and 6.61mg/mL, respectively. The experiments indicated that the immobilization increased the thermal stability of the -amylase. The immobilized -amylase, easily separated from the reaction media, could be used effectively for 15 cycles with 65% retention of the initial activity. Moreover, the immobilized -amylase was effectively used in packed bed bioreactor for continuous starch hydrolysis throughout 6.5h without loss of activity. Different starches (e.g., potato and wheat) were hydrolyzed by the immobilized -amylase in continuous process. The results indicate a potential use of this immobilized enzyme system for the construction of packed bed bioreactors to be used in the hydrolysis of the starch.
Açıklama
Temocin, Zulfikar/0000-0001-7151-9772
Anahtar Kelimeler
Amylase, Hydrolysis, Immobilization, Packed bed bioreactor, Starch
Kaynak
Starch-Starke
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
66
Sayı
3-4
Künye
closedAccess
