Immobilization of a- amylase on reactive modified fiber and its application for continuous starch hydrolysis in a packed bed bioreactor

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dc.contributor.authorTemocin, Zuelfikar
dc.date.accessioned2020-06-25T18:12:17Z
dc.date.available2020-06-25T18:12:17Z
dc.date.issued2014
dc.departmentKırıkkale Üniversitesi
dc.descriptionTemocin, Zulfikar/0000-0001-7151-9772
dc.description.abstractIn this study, the enzyme -amylase was immobilized on reactive modified poly (ethylene terephthalate) fiber. The activities of free -amylase and immobilized -amylase were compared in a batch starch hydrolysis system. Optimum pH and Michaelis-Menten constants were determined for both free -amylase and immobilized -amylase. The immobilization shifted the optimum pH to a higher level. The Michaelis-Menten constant values (K-m) of the immobilized -amylase and free -amylase were obtained as 11.94 and 6.61mg/mL, respectively. The experiments indicated that the immobilization increased the thermal stability of the -amylase. The immobilized -amylase, easily separated from the reaction media, could be used effectively for 15 cycles with 65% retention of the initial activity. Moreover, the immobilized -amylase was effectively used in packed bed bioreactor for continuous starch hydrolysis throughout 6.5h without loss of activity. Different starches (e.g., potato and wheat) were hydrolyzed by the immobilized -amylase in continuous process. The results indicate a potential use of this immobilized enzyme system for the construction of packed bed bioreactors to be used in the hydrolysis of the starch.en_US
dc.description.sponsorshipKirikkale University Research FundKirikkale Universityen_US
dc.description.sponsorshipI am grateful to the Kirikkale University Research Fund for the financial support for this work (2011-2021).en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/star.201300132
dc.identifier.endpage384en_US
dc.identifier.issn0038-9056
dc.identifier.issn1521-379X
dc.identifier.issue3-4en_US
dc.identifier.scopus2-s2.0-84900518686
dc.identifier.scopusqualityQ2
dc.identifier.startpage376en_US
dc.identifier.urihttps://doi.org/10.1002/star.201300132
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5845
dc.identifier.volume66en_US
dc.identifier.wosWOS:000332335900017
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWiley-V C H Verlag Gmbhen_US
dc.relation.ispartofStarch-Starke
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmylaseen_US
dc.subjectHydrolysisen_US
dc.subjectImmobilizationen_US
dc.subjectPacked bed bioreactoren_US
dc.subjectStarchen_US
dc.titleImmobilization of a- amylase on reactive modified fiber and its application for continuous starch hydrolysis in a packed bed bioreactoren_US
dc.typeArticle

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