Escherichia coli in different animal feces: phylotypes and virulence genes

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dc.authoridKarakaya, Emre/0000-0003-2390-6190
dc.contributor.authorKarakaya, Emre
dc.contributor.authorAydin, Fuat
dc.contributor.authorKayman, Tuba
dc.contributor.authorAbay, Secil
dc.date.accessioned2025-01-21T16:40:35Z
dc.date.available2025-01-21T16:40:35Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractIn this study, it was aimed to determine the phylogroups of Escherichia coli isolates from horse, cat, dog, sheep, cattle, and chicken feces samples and to investigate some important virulence genes of the isolates. For this purpose, a total of 600 feces samples, 100 from each animal species, were used as material. For the isolation of E.coli, feces samples were directly inoculated on MacConkey agar. The identification of the isolates was performed via phenotypic tests and species-specific multiplex Polymerase Chain Reaction (mPCR) method. PCR methods were used to phylotype E.coli isolates and to investigate virulence genes (bfpA, eaeA, LT, ST, Stx1, and Stx2). Of the total 600 E.coli isolates recovered in this study, 120 (20%), 269 (44.8%), 58 (9.7%), 19 (3.2%), 35 (5.8%), 56 (9.3%), 31 (5.2%), and 12 (2%) were identified as phylogroup A, B1, B2, C, D, E, F, and Escherichia clade I, respectively. While the virulence gene was detected in 149 (24.8%) E.coli isolates, no virulence gene was detected in 451 (75.2%) isolates. According to the analysis results, the most determined virulence gene was Stx1, while the least determined virulence gene was LT. In conclusion, in this study, when both the animal species and the number of E.coli isolates examined are considered, the data obtained are of great importance in epidemiological terms. However, the detection of virulence genes in 13.5% among phylogroup A, B1, and C isolates with commensal characteristics suggest that these isolates may show pathogenic characteristics with the virulence genes they contain.
dc.identifier.doi10.1007/s11274-022-03451-w
dc.identifier.issn0959-3993
dc.identifier.issn1573-0972
dc.identifier.issue1
dc.identifier.pmid36383288
dc.identifier.scopus2-s2.0-85141921860
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s11274-022-03451-w
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24704
dc.identifier.volume39
dc.identifier.wosWOS:000884730100001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofWorld Journal of Microbiology & Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectIdentification; Escherichia coli; Feces; Phylogroup; Virulence genes
dc.titleEscherichia coli in different animal feces: phylotypes and virulence genes
dc.typeArticle

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