High-fructose consumption suppresses insulin signaling pathway accompanied by activation of macrophage and apoptotic markers in rat testis

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dc.authoridAkar, Fatma/0000-0002-5432-0304
dc.authoridGUNEY, Ceren/0000-0002-3267-2886
dc.contributor.authorYildirim, Onur Gokhan
dc.contributor.authorGuney, Ceren
dc.contributor.authorAlcigir, Mehmet Eray
dc.contributor.authorAkar, Fatma
dc.date.accessioned2025-01-21T16:41:27Z
dc.date.available2025-01-21T16:41:27Z
dc.date.issued2023
dc.departmentKırıkkale Üniversitesi
dc.description.abstractDietary high-fructose may cause metabolic disturbances; however, its effect on the reproductive system is little understood. The insulin signaling pathway is critical in testicular development, maintenance of microcirculation and spermatogenesis. Therefore, in this study, we aimed to investigate the impact of dietary high-fructose on insulin signaling pathway as well as macrophage and apoptotic markers in testicular tissue of rats. Fructose was administered to male Wistar rats as a 20% solution in drinking water for fifteen-week. Gene expression of ir-beta, irs-1, irs-2, pi3k, akt, mtor, and enos in the testicular samples was determined by real-time PCR. Protein expression of IR, IRS-1, IRS-2, PI3K, Akt, phospho-Akt (p-Akt), mTOR, eNOS, phospho-eNOS (p-eNOS), and GLUT5 was established by analysis of Western Blot. Testicular expression of occludin, CD163, CD68, caspase-8, and caspase-3 was analyzed by using immunohistochemical assay. Testicular level of fructose was measured by colorimetric method. Dietary high-fructose decreased mRNA expressions of irs-1, irs-2, pi3k, and mtor in the testicular tissue of rats. Also, this dietary intervention impaired protein expressions of IR, IRS-1, IRS-2, PI3K, p-Akt, mTOR, eNOS, and p-eNOS as well as p-Akt/Akt and p-eNOS/eNOS ratios in the testis of rats. However, a high-fructose diet increased the expression of CD163, CD68, caspase-8 and caspase-3, but decreased that of occludin, in the testicular tissue of rats. The high-fructose consumption in rats suppresses testicular insulin signaling but activates macrophages-related factors and apoptotic markers. These changes induced by dietary fructose could be related to male reproductive dysfunction.
dc.description.sponsorshipGazi University
dc.description.sponsorshipFunding This study was supported by grants from the Gazi University
dc.identifier.doi10.1016/j.repbio.2023.100815
dc.identifier.issn1642-431X
dc.identifier.issn2300-732X
dc.identifier.issue4
dc.identifier.pmid37839228
dc.identifier.scopus2-s2.0-85174030212
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.repbio.2023.100815
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24891
dc.identifier.volume23
dc.identifier.wosWOS:001097283800001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofReproductive Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectDietary fructose; Insulin signaling pathway; Macrophages; Apoptosis; Rat testis
dc.titleHigh-fructose consumption suppresses insulin signaling pathway accompanied by activation of macrophage and apoptotic markers in rat testis
dc.typeArticle

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