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Öğe Cadmium Induced Changes on Growth Performance, Some Biochemical Parameters and Tissue in Broilers: Effects of Vitamin C and Vitamin E(Academic Journals Inc, 2011) Cinar, Miyase; Yigit, A. Arzu; Yalcinkaya, Ilkay; Oruc, Ertan; Duru, Ozkan; Arslan, MetinThis study was performed to determine the effects of vitamin C (vit C) and vitamin E (vit E) on growth performance, some biochemical parameters and tissue changes in broiler chickens exposed to cadmium (Cd). One hundred twenty, 1-day-old, Ross broiler chicks were divided into 4 groups with 3 replicates and fed with a basal diet (control) or the basal diet supplemented with 60 mg kg(-1) Cd, 60 mg kg(-1) Cd+400 mg kg(-1) vit C, or 60 mg kg(-1) Cd+250 mg kg(-1) vit E for 42 days. Body weight (BW), Body Weight Gain (BWG) and Feed Consumption (FC) were recorded and feed conversion ratio (FCR) was calculated. Plasma were analysed for aspartate transaminase (AST), alanine transaminase (ALT), gamma glutamyltransferase (GGT) activities and total protein, total cholesterol and creatinine levels. Cadmium significantly decreased the BW (p<0.001), BWG (p<0.001), FC (p<0.001) and FCR (p<0.05) compared with the control group. Regarding the same groups plasma and kidney Cd (p<0.001) and AST (p<0.05) and GGT activities (p<0.01) were increased. While growth performance were not affected in vit C and vit E supplementated group compared with the Cd-only group, these treatments reduced plasma Cd and GGT activities. Furthermore vit C was more effective in reducing Cd accumulation in kidneys and liver.Öğe Characterization of chondrocytes cultured on catechin-loaded alginate-chitosan scaffolds(Informa Healthcare, 2013) Turk, Mustafa; Karahan, Siyami; Cinar, Miyase; Kucuk, Sebnem; Dincel, Gungor CagdasBovine chondrocytes were seeded into scaffolds of a high molecular weight chitosan and alginate with a pore size of 50-350 mu m with or without catechin. In polymerase chain reaction (PCR), unlike type II, collagen type I was no longer expressed at day 14. The DNA content increased until day 8 and began declining, indicating cell detachment. The GAG content increased during the first 12 days. The percentage of round and collagen type II immunoreactive cells increased over the time. Catechin has some protective properties on chondrocytes seeded on the alginate-chitosan scaffolds during the first 12 days by means of DNA and chondrocyte morphology (p < 0.05).Öğe Determination of antioxidants in bovine oviduct epithelial cell culture isolated at different periods of the estrous cycle(Scientific Technical Research Council Turkey-Tubitak, 2019) Kurum, Aytul; Karahan, Siyami; Kocamis, Hakan; Cinar, Miyase; Ergun, EmelOxidative stress interferes with oviduct functions including oocyte maturation, capacitation, fertilization, and embryo and gamete transport. This study aimed to determine activity of the antioxidants glutathione peroxidase (GPX-I), superoxide dismutase (SOD), and catalase (CAT) in bovine oviduct epithelial cells (BOEC) isolated from the isthmus and ampulla of the oviduct at estral (n = 7) and luteal phases (n = 7) of the estrous cycle. The antioxidant activity was measured at the primary, first, and second passages of the cell culture, and was characterized by cytokeratin expression. The GPX activity increased over the passages in samples of the ampulla and the isthmus of each sexual phase without statistical significance. The SOD activity remained steady through the cell passages in both sexual phases. CAT activity at the primary culture was higher in the ampulla compared to the isthmus in both sexual phases with a significant difference for the estral phase (P < 0.05), and it decreased over the passages with no significant differences. In conclusion, the antioxidant enzyme activity profile of BOEC did not differ by region or sexual cycle except for that of CAT, which was higher in the ampulla. Further studies should focus on SOD, GPX, and CAT activity for the mechanism of BOEC adaptation to an in vitro environment.Öğe The Effect of Chlorpyrifos on Isolated Thoracic Aorta in Rats(Hindawi Ltd, 2013) Yildirim, Ebru; Baydan, Emine; Kanbur, Murat; Kul, Oguz; Cinar, Miyase; Ekici, Husamettin; Atmaca, NurgulThis study investigated the effect of chlorpyrifos on thoracic aorta and on the level of NO in plasma and aorta. The effect of chlorpyrifos on thoracic aorta in organ bath was determined in 10 rats. Another 45 rats were assigned to 3 groups with 15 rats each: control group 1 received distilled water, control group 2 was given corn oil, and the last group was given 13.5 mg/kg chlorpyrifos dissolved in corn oil every other day for 8 weeks orally. Chlorpyrifos (10(-10) M-10(-5) M) showed no effect on isolated thoracic aorta. Plasma AChE activity was decreased, while LDH, ALT, GGT, and AST activities were increased in chlorpyrifos group compared to control groups. Plasma NO level was increased in chlorpyrifos group compared to control groups. iNOS expression was present in all groups in the cytoplasm of the endothelia and in the smooth muscle cells of aorta. According to semiquantitative histomorphological analysis, iNOS immunopositive reactions were seen in the decreasing order in chlorpyrifos, control 2, and control 1 groups. eNOS immunopositive reactions were observed in the endothelial cell cytoplasm, rarely in the subintimal layer, and the smooth muscle cells of aorta. There were no differences among the groups in terms of eNOS immunostaining. In conclusion, chlorpyrifos induced NO production in aorta following an increase in NOS expression.Öğe Effect of Cocoa Butter and Sunflower Oil Supplementation on Performance, Immunoglobulin, and Antioxidant Vitamin Status of Rats(Hindawi Publishing Corporation, 2014) Yildirim, Ebru; Cinar, Miyase; Yalcinkaya, Ilkay; Ekici, Husamettin; Atmaca, Nurgul; Guncum, EnesThis study investigated the effects of cocoa butter and sunflower oil alone and in combination on performance, some biochemical parameters, immunoglobulin, and antioxidant vitamin status in Wistar rats. Forty-eight male rats were assigned to four groups, consisting of 12 rats with 3 replicates. Control received balanced rat diet without oil, cocoa butter group received 3.5% cocoa butter, sunflower oil group received 3.5% sunflower oil, the last group received 1.75% sunflower oil + 1.75% cocoa butter supplementation in the rat diet for 8 weeks. The total feed consumption in sunflower oil group was statistically lower than in the other groups. The serum creatinine level was decreased in cocoa butter group compared to control. Triglyceride and VLDL cholesterol levels were decreased in only sunflower oil and only cocoa butter groups as compared to control. The level of Ig M was statistically lower in cocoa butter and cocoa butter + sunflower oil groups than in control and sunflower oil groups. There were no statistically important difference in vitamin concentrations among trial groups. It was concluded that the supplementation of cocoa butter in diet decreased Ig M level, while the supplementation of cocoa butter and sunflower oil alone decreased the triglyceride and VLDL cholesterol levels.Öğe The Effect of Organic Selenium and Vitamin E on Performance, Internal Organ Weight and Blood Parameters in Broiler Rations(Kafkas Univ, Veteriner Fakultesi Dergisi, 2010) Yalcinkaya, Ilkay; Gungor, Tulin; Basalan, Mehmet; Cinar, Miyase; Sacakli, PinarThis study was conducted to investigate the effects of rations containing organic selenium and Vitamin E on live weight, live weight gain, feed consumption, feed efficiency, internal organ weights and blood parameters of broiler chicks. A total of 120, one day old Ross 308 male broiler chicks were used in this study. There were 4 treatment groups each containing 10 chicks of 3 replicates. The control group (K) was fed a basal diet without supplemented organic selenium (Se) and Vitamin E. Treatment groups were fed with 0.6 ppm organic selenium (Sel-Plex) (Se); 150 IU/kg Vitamin E (E) and 0.6 ppm organik selenium (Sel-Plex) + 150 IU Vitamin E (Se+E). The experiment lasted 42 days. There were no significant differences live weight, feed consumption, feed efficiency and relative internal organ weight among the groups (P>0.05). Serum vitamin E levels were higher in vitamin E (E) supplemented group than in other groups (P<0.05).Öğe The effect of prebiotic and organic zinc alone and in combination in broiler diets on the performance and some blood parameters(Taylor & Francis Ltd, 2012) Yalcinkaya, Ilkay; Cinar, Miyase; Yildirim, Ebru; Erat, Serkan; Basalan, Mehmet; Gungor, TulinThis study was conducted to investigate the effects of prebiotic and organic zinc alone and in combination in broiler diets on the live weight gain (LWG), feed consumption (FC), feed consumption ratio (FCR), carcass yield, some relative organ weights and blood parameters. A total of 160 Ross 308 one-day old male chicks were assigned to 4 treatment groups with 4 replicates of 10 birds each. Treatment for each group consisted of: first group (control group) received basal diet without supplementation; second group received 1 g/kg Mannano ligosaccharide (MOS); third group received 80 mg/kg organic zinc (OZn); and fourth group received 80 mg/kg organic zinc + 1 g/kg Mannanoligosaccharide (MOS+OZn). The study lasted 42 days. The supplementation of MOS and OZn had no effect on the LWG, FC, FCR, carcass yield, serum aspartate aminotranferase, (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and triglyceride levels during the experiment (P>0.05). Relative organ weights (liver, spleen, pancreas) were significantly higher in OZn group than those in the other groups (P<0.001). There was a significant difference in serum total cholesterol and glucose levels between treatment groups and control group (P<0.001). Serum Zn and Fe levels were significantly lower in control group than those in OZn and MOS+OZn groups (P<0.001). The highest and the lowest Cu levels were in the MOS+OZn and control groups, respectively (P<0.001). These data suggest that OZn with MOS combination may have a beneficial effect on serum mineral level in broilers.Öğe Effects of defaunation and dietary nitrogen source on sodium, potassium, iron and zinc in the rumen fluid, plasma and wool of lambs(Scientific Technical Research Council Turkey-Tubitak, 2006) Ozdemir, Mehmet; Cinar, Miyase; Haliloglu, Seyfullah; Eryavuz, AbdullahThis study was conducted to investigate the effects of defaunation, and the addition of urea and sulfur in the replacement of plant protein (PP) sources on concentrations of Na, K, Zn and Fe in the ruminal fluid, blood plasma and wool of lambs. Forty male Ramlic lambs, 2.70 +/- 0.30 months of age, and weighing approximately 16.44 +/- 0.41 kg, were used. Lambs were divided into 4 groups as follows: faunate + control diet (FC), defaunate + control diet (DC), faunate + experimental diet (FE), and defaunate + experimental diet (DE). The control diet contained plant protein as a N source, whereas the experimental diet was supplemented with urea and sulfur, both at 3% of BW per day. The forage portion of the diet was 350 g of alfalfa hay per lamb per day. Diets were fed twice daily in equal amounts. Defaunation, or feeding a diet supplemented with urea and sulfur in place of plant protein sources, had no effect on ruminal fluid Na concentrations but there was an interaction on Na concentration in the rumen fluid. Ruminal K concentration was lower (P < 0.021) in the experimental diet versus the control diet, while it was not affected by defaunation. There was an interaction effect on Zn concentration in rumen fluid. Plasma Na, K and Zn concentrations were not affected by treatments, whereas defaunation significantly decreased (P < 0.048) plasma Fe concentration. Defaunation or N source had no effect on Na, K, Zn and Fe concentrations in wool. We concluded that both defaunation and feeding a diet supplemented with urea and sulfur in place of plant protein sources did not affect blood plasma, except for Fe, and wool Na, K, Zn and Fe concentrations despite differences in their ruminal concentration.Öğe Effects of Dietary Supplementation with Vitamin C and Vitamin E and Their Combination on Growth Performance, Some Biochemical Parameters, and Oxidative Stress Induced by Copper Toxicity in Broilers(Humana Press Inc, 2014) Cinar, Miyase; Yildirim, Ebru; Yigit, A. Arzu; Yalcinkaya, Ilkay; Duru, Ozkan; Kisa, Ucler; Atmaca, NurgulThis study investigated effects of dietary supplementation with vitamin C, vitamin E on performance, biochemical parameters, and oxidative stress induced by copper toxicity in broilers. A total of 240, 1-day-old, broilers were assigned to eight groups with three replicates of 10 chicks each. The groups were fed on the following diets: control (basal diet), vitamin C (250 mg/kg diet), vitamin E (250 mg/kg diet), vitamin C + vitamin E (250 mg/kg + 250 mg/kg diet), and copper (300 mg/kg diet) alone or in combination with the corresponding vitamins. At the 6th week, the body weights of broilers were decreased in copper, copper + vitamin E, and copper + vitamin C + vitamin E groups compared to control. The feed conversion ratio was poor in copper group. Plasma aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activities, iron, copper concentrations, and erythrocyte malondialdehyde were increased; plasma vitamin A and C concentrations and erythrocyte superoxide dismutase were decreased in copper group compared to control. Glutathione peroxidase, vitamin C, and iron levels were increased; aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and copper levels were decreased in copper + vitamin C group, while superoxide dismutase, glutathione peroxidase, and vitamin E concentrations were increased; aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase were decreased in copper with vitamin E group compared to copper group. The vitamin C concentrations were increased; copper, uric acid, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and malondialdehyde were decreased in copper + vitamin C + vitamin E group compared to copper group. To conclude, copper caused oxidative stress in broilers. The combination of vitamin C and vitamin E addition might alleviate the harmful effects of copper as demonstrated by decreased lipid peroxidation and hepatic enzymes.Öğe Effects of magnesium sulphate on the oxidative stress, biochemical and hematological parameters in pregnant rats with L- NAME induced preeclampsia(Parlar Scientific Publications (P S P), 2011) Cinar, Miyase; Yildirim, Ebru; Yigit, A. Arzu; Macun, H. Ceyhun; Duru, OzkanThe aim of this study was to investigate the effects of magnesium sulphate (MgSO4) on some biochemical and hematological parameters, lipid peroxidation, and antioxidant enzymes in pregnant rat model of preeclampsia induced by L-w-nitro-L-arginine methyl ester (L-NAME). Thirty two pregnant Sprague-Dawley rats were divided into 4 equal groups as control, L-NAME (50 mg/kg bw/day), MgSO4 (600 mg/kg bw/day) or L-NAME + MgSO4 (1-4) given groups on the 11(th) day of the pregnancy in their drinking water. The increased blood pressure (BP) was detected on the 12(th) and 19(th) days of pregnancy. Blood samples were collected on the 19(th) day to determine blood oxidative status, some biochemical and hematological parameters. L-NAME decreased erythrocyte superoxide dismutase activity, plasma total protein level and increased erythrocyte catalase and glutathion peroxidase activities, BP, plasma malondialdehyde, cholesterol and triglyceride levels, alkaline phophatase activity and white blood cell counts compared to control group. While MgSO4 decreased aspartate amino transferase and alanine amino transferase activities and calcium level, it increased magnesium level compared to control group. Increased BP, plasma cholesterol levels, erythrocyte catalase and glutathione peroxidase activities by L-NAME were decreased by the addition of MgSO4. In conclusion, MgSO4 (600 mg/kg bw/day) may be beneficial in the treatment of preeclampsia by increasing usage of antioxidant enzymesÖğe Effects of Supplemental Epigallocatechin Gallate in the Diet of Broilers Exposed to Fluoride Intoxication(Humana Press Inc, 2018) Aydogan, Ilkay; Karsli, Mehmet Akif; Basalan, Mehmet; Yildirim, Ebru; Cinar, Miyase; Sen, Goekhan; Sumer, TugceWe evaluated the effects of dietary epigallocatechin gallate (EGCG) on the performance, biochemical parameters, and liver histopathology of fluoride-intoxicated broiler chickens. In total, 160 1-day-old male broiler chicks (Ross PM3 strain) were collected and assigned to four groups (40 animals each), with four replicates. The control group received a basal diet; the F group received 800mg/kg fluoride; the EGCG group received 400mg/kg EGCG; and the EGCG+F group received 400mg/kg EGCG and 800mg/kg fluoride. The live weight (LW) of F-treated chicks was significantly lower than that of the controls. In the F-treated groups, feed intake (FI) and LW values were lower, but the feed conversion ratio (FCR) was higher than those of the controls. The ratio of heart weight to LW was found to be the highest in the F-treated groups. Alkaline phosphatase (ALP), aspartate aminotransferase (AST), and total oxidant status (TOS) levels in the F-treated groups were significantly higher, whereas the increase in total cholesterol levels was insignificant than those in the control group. In the EGCG+F group, AST, total cholesterol, and TOS levels decreased to a level comparable to those in the control group. Histopathological evaluation revealed that there were mild changes in the portal region in the EGCG+F group; additionally, there was an improvement in liver morphology in the EGCG+F group compared to that in the F group. Thus, EGCG has potent antioxidant and regenerative effects that can ameliorate the detrimental effects of fluoride toxicity on blood parameters and the liver.Öğe The Effects of Yeast Glucomannan (Mycosorb) on Lipid Peroxidation and Non-Enzymatic Antioxidative Status in Experimentally Induced Aflatoxicosis in Broilers(Medwell Online, 2008) Cinar, Miyase; Yildirim, Ebru; Yalcinkaya, Ilkay; Eraslan, GoekhanThe aim of this study was to determine the effect of yeast glucomannan (Mycosorb), an aflatoxin binder, on lipid peroxidation and non-enzymatic antioxidative status in experimentally induced aflatoxicosis in broilers. Forty, I-day-old male broiler (Ross 308) chickens were randomly divided into 4 groups of 10 birds each. The control group was fed a basal diet and the remaining groups received 0.75 g kg(-1) Mycosorb, 2 ppm Aflatoxin (AF) and 0.75 g kg(-1) Mycosorb +2 ppm Aflatoxin (AF) for 21 days. At the end of day 21, blood samples were collected and plasma Malondialdehyde (MDA), Ceruloplasmin (CP), albumin (alb), uric acid, vitamin A (vit A), beta-carotene, vitamin C (vit C) and vitamin E (vit E) levels were determined. Plasma MDA levels increased insignificantly and A levels decreased significantly in AF and AF+Mycosorb groups when compared to control and mycosorb groups. Plasma CP, vit A and beta-carotene levels decreased significantly in AF groups when compared to control. In AF and AF+Mycosorb group, significant decreases were determined in vit E levels when compared to control and in vit C levels when compared to mycosorb group. Plasma uric acid levels were not affected by these treatments. It is concluded that subacute dietary intake of AF altered nonenzymatic components (CP, alb, vit A, beta carotene, Vit E) of antioxidant defense systems and 0,75 g kg(-1) yeast glucomannan was not sufficient to ameliorate the oxidative damage caused by AF in broilers.Öğe Evaluation of biochemical parameters and oxidative stress in sheep naturally infected with Dicrocoelium dendriticum and hydatid cysts(Scientific Technical Research Council Turkey-Tubitak, 2018) Cinar, Miyase; Aydenizoz, Meral; Gokpinar, Sami; Camkerten, GuzinThe purpose of the study was to investigate the lipid peroxidation and antioxidant status as well as biochemical profile in sheep naturally infected with Dicrocoelium dendriticum and hydatid cysts. Ten sheep naturally infected with D. dendriticum, 10 sheep naturally infected with hydatid cysts, and 10 healthy sheep were used. The blood and visceral organs of sheep brought to a slaughterhouse in the region of Kirikkale were checked for D. dendriticum and hydatid cysts. Plasma total protein, albumin, total bilirubin, total cholesterol, Ca, Mg, inorganic P, Cu, Zn, vitamin A, vitamin C, vitamin E, beta-carotene, AST, ALT, GGT, ALP, erythrocyte MDA levels, and SOD and CAT activities were detected. Erythrocyte MDA, plasma total bilirubin levels, and AST and ALP activities were increased while total protein and vitamin A levels were decreased in sheep infected with parasites. Erythrocyte SOD activities were decreased in D. dendriticum group, and CAT activities were decreased in the hydatid cyst group and D. dendriticum group. Total bilirubin levels were increased and total protein levels were decreased in sheep infected with parasites. These results suggest that endoparasitic infections such as D. dendriticum and hydatid cysts cause oxidative stress and change some biochemical parameters in sheep.Öğe Evaluation of oxidative stress, hematological and biochemical parameters during Toxoplasma gondii infection in gerbils(Ankara Univ Press, 2015) Atmaca, Nurgul; Cinar, Miyase; Guner, Bayram; Kabakci, Ruhi; Gazyagci, Aycan Nuriye; Atmaca, Hasan Tarik; Canpolat, SilaThe aim of the present study was to investigate the alterations of oxidative stress, hematological and biochemical parameters in experimental infection caused by Toxoplasma gondii in gerbil. A total of 16 gerbil, 8 of which were control and 8 was infection group, were used in the study. The gerbils were infected by intraperitoneal inoculation of 5000 T. gondii RH strain tachyzoites. In group of, the gerbil were sacrificed at 7th day after inoculation. At the end of this period, blood samples collected and erythrocyte malondialdehyde (MDA) concentrations, superoxide dismutase (SOD), catalase (CAT) activities, plasma aspartat aminotransferase (AST) and alanine aminotranspherase (ALT) activities, total protein, albumin, globulin were determined. Besides, hematological parameters were analysed in whole blood. Aspartat aminotransferase and ALT activities and MDA concentrations and neutrophil percentage and total leukocyte counts increased significantly in infected group when compared to control. In infected group, SOD activities, albumin concentrations and lymhocyte percentage decreased when compared to control. The results of this study suggest that oxidative stress, hematological and biochemical alterations may contribute to the pathogenesis of toxoplasmosis in gerbils.Öğe Heavy metal accumulation and oxidative-antioxidative status in Angora goats depending on age(Parlar Scientific Publications (P S P), 2015) Simsek, Ozkan; Ekici, Husamettin; Cinar, Miyase; Atmaca, Nurgul; Arikan, Sevket; Guner, BayramThis study intends to designate the alterations in the oxidant-antioxidant parameters and the heavy metal concentrations based on age of Angora goats bred in Cankiri-province of Turkey. In order to conduct an analysis of serum heavy metals including aluminum (Al), chromium (Cr), iron (Fe), copper (Cu), cobalt (Co), tin (Sn), cadmium (Cd), selenium (Se), vanadium (V), manganese (Mn), zinc (Zn), lead (Pb), arsenic (As), nickel (Ni), a total of 120 blood samples were taken. Concentrations of plasma vitamin E, vitamin A and beta-carotene, concentration of malondialdehyde (MDA), activities of catalase (CAT) and superoxide dismutase (SOD) were evaluated in the erythrocytes. Correspondingly, compared to young goats, the adult goats had substantially higher levels of Al, V, Mn, Ni, As and Sn. With respect to the young goats, the vitamin E concentration and SOD activity measured in adult goats were lower and the MDA concentration was considerably higher. Consequently, it was seen that aging affected the oxidant-antioxidant parameters and heavy metal concentrations in Angora goats.Öğe Hematobiochemical profiles, mineral concentrations and oxidative stress indicators in beef cattle with pica(Taylor & Francis Ltd, 2019) Onmaz, Ali Cesur; Gunes, Vehbi; Cinar, Miyase; Citil, Mehmet; Keles, IhsanPica, or "depraved appetite", is defined as ingestion of non-nutritive substances. Many factors influencing pica in animals have been identified , but the etiology is controversial. This study was conducted to evaluate haematological and biochemical parameters, trace element concentrations, the total antioxidant status (TAS), total oxidative status (TOS) and oxidative stress index (OSI) in beef cattle with and without pica. Ten beef cattle with pica (group I) and another ten healthy beef cattle (Group II) were used in the study. Serum TAS and TOS were measured by a colorimetric method. In group I, haemoglobin value and serum iron, copper and selenium concentrations were significantly lower than those of group II (p < .05). Cortisol level and the other biochemical parameters were not statistically different between the groups (p > .05). Compared to the healthy controls mean TAS values were significantly decreased in the group I (p < .05), whereas the TOS and OSI values did not differ statistically between the two groups (p > .05). Parasitological analysis revealed that Trichostrongylidae spp eggs were more common in group I. In conclusion, serum iron, copper and selenium deficiency with decreased antioxidant capacity may cause the symptoms of pica in beef cattle. Prophylactic use of trace mineral supplement containing iron, copper, selenium and antioxidants in beef cattle may be beneficial in preventing deviated appetite and in strengthening the antioxidant defence system.Öğe Hepatogenous photosensitization in Akkaraman lambs: special emphasis to oxidative stress and thrombocytopenia(Ankara Univ Press, 2013) Ocal, Naci; Haydardedeoglu, Ali Evren; Cinar, Miyase; Kul, Oguz; Turk, MustafaIn this study, a total of 24 Akkaraman lambs with clinical signs that are reminiscent of photosensitization composed the experimental group (Group I). Additional 10 healthy lambs were included as controls (Group II). We were aimed to make definitive diagnosis of photosensitization, which can be confused with blue tongue, big head and sunburns and to establish etiology as primary or hepatogenous. In total blood analyses, the leukocyte count was higher, but thrombocyte count was lower (thrombocytopenia) in Group I compared to Group II. The lower MCV indicates presence of a case of microcytosis. Furthermore, affected lambs had significantly higher levels of phylloerythrin, gamma-glutamyl transpeptidase, aspartate aminotransferase, alkaline phosphatase, cholesterol and serum urea nitrogen. Histopathological lesions included periaciner necrosis, periportal fibrosis and biliary duct hyperplasy. The grazing field, where the lambs had been grazing, was very rich in Tribulus terrestris, a hepatotoxic plant. Based on the increased levels of phylloerythrin, gamma-glutamyl transpeptidase, aspartate aminotransferase and alkaline phosphatase as well as pathological findings in liver, the cases were diagnosed as hepatogenous photosensitization. In addition, presence of Tribulus terrestris in the grazing pasture strongly supported the diagnosis. Increased levels of serum malondialdehyde in affected lambs showed an ongoing oxidative stress. In addition, thrombocytopenia in such cases should be considered as a risk factor for disseminated intravascular coagulation (DIC). Thus in addition to a semptomatical treatment protocol, use of antioxidants, anticoagulants, and liver protective agents shoud be taken into consideration in hepatogenous photosensitization in lambs.Öğe Oxidant and antioxidant status of human breast milk during lactation period(Springer France, 2015) Yuksel, Sevda; Yigit, Ayse Arzu; Cinar, Miyase; Atmaca, Nurgul; Onaran, YukselDue to the oxidative stress conditions at birth, newborn needs some immunological and antioxidative protection after birth. The present study was conducted to point out the oxidant and antioxidant activity of human breast milk throughout the lactation. For this purpose, the levels of malondialdehyde, nitric oxide, lactoferrin, superoxide dismutase, and catalase was determined in colostrum, transition milk, and mature milk. Milk samples were collected from 20 volunteer mothers at 24-48 h of delivery and 7 and 14 days after delivery. The skimmed milk was used for determination of the levels of malondialdehyde, nitric oxide, and lactoferrin and the activities of the catalase and superoxide dismutase enzymes. As the lactation period proceeded, the malondialdehyde level increased and the levels of nitric oxide and lactoferrin as well as the activities of catalase and superoxide dismutase decreased. Colostrum has a high antioxidant capacity, and these antioxidants decrease during the lactation period owing to the changing need of the growing infant. The results of this study revealed the importance of breastfeeding immediately after the birth to take the advantage of colostrum.Öğe Reference values for selected hematological and biochemical blood parameters from prepregnancy to advanced gestation in Angora cats(Scientific Technical Research Council Turkey-Tubitak, 2015) Simsek, Ozkan; Arikan, Sevket; Cinar, MiyaseThe aim of this study was to monitor some hematological and biochemical blood parameters from prepregnancy to advanced gestation in Angora cats. In the present study, 35 blood samples obtained before and during pregnancy from 7 adult cats were used. Blood was collected from cats before pregnancy and on days 15, 30, 45, and 55 of gestation. The blood samples were analyzed in terms of WBC, RBC, Hb, PCV, MCV, MCH, MCHC, GRA, MON, and LYM values. Additionally, serum ALT, AST, ALP, GGT, LDH, and CK activities and total cholesterol, glucose, triglyceride, urea, Ca, Mg, and Pi levels were measured. Levels of leukocytes, MCHC, MCH, and MCV were lower, and RBC, PCV, and Hb levels were higher before pregnancy than on day 55 of gestation. Monocyte and glucose levels were higher before gestation than during pregnancy. Total cholesterol levels were lower before gestation than during gestation. Triglyceride and Mg levels with LDH activity were higher in the late stages of pregnancy than before gestation. In conclusion, in this study changes in selected hematological and biochemical blood parameters have been determined before and during pregnancy in Angora cats for the first time. The data presented in this research may be a useful reference for veterinarians practicing in clinics.Öğe The impact of bone marrow-derived mesenchymal stem cells on experimental testicular torsion in rats(Tubitak Scientific & Technological Research Council Turkey, 2022) Erturk, Ahmet; Demir, Sabri; Gunal, Yasemin Dere; Zengin, Mehmet; Cinar, Miyase; Yildiz, Dincer; Karahan, SiyamiBackground/aim: The aim of this study was to investigate the healing effects of bone marrow-derived mesenchymal stem cells (BM-MSCs) on experimental testicular torsion in rats. Materials and methods: Three groups consisting of 10 Wistar albino rats were created. In Group I, the left testicle was explored and relocated in the scrotum without any attempt to modify it. In Group II, the left testicle underwent torsion for three h and then was detorsed and relocated. In Group III, in addition to torsion and detorsion, BM-MSCs were administered intratesticularly. The rats were sacrificed on the seventh day, and the healing status of the testicles was investigated with histopathological and biochemical analyses. BM-MSC involvement was investigated by immunofluorescence microscopy. Statistical analysis was performed using SPSS 15.0. A p-value < 0.05 was considered statistically significant for all variables. Results: Immunofluorescence microscopy showed that BM-MSCs were located around the Leydig cells in Group III. Under light microscopy, the mean Johnsen Score of Group III was significantly higher than that of Group II (p = 0.035). The interleukin-10 (IL-10) level was significantly higher in Group III compared to Group II (p = 0.003). While the malondialdehyde (MDA) values in Group I (the control group) were lower than in the other groups (p = 0.037), the superoxide dismutase (SOD) values were similar (p = 0.158). Although there was no statistically significant difference between Group II and Group III in terms of MDA, it was lower in Group III. Although the tissue SOD levels were higher in Group III than in Group II, the difference was not statistically significant. Conclusion: This study has demonstrated that BM-MSCs significantly corrected the Johnsen Score and increased anti-inflammatory cytokine levels after testicular torsion. BM-MSCs can be used in testicular torsion as supportive therapy to minimize tissue damage.
