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Öğe A detailed study on multifaceted bioactivities of the extracts and isolated compounds from truffle Reddellomyces parvulosporus(Wiley, 2022) Cayan, Fatih; Tel-Cayan, Gulsen; Deveci, Ebru; Duru, Mehmet Emin; Turk, MustafaMushrooms and truffles are attracting attention as a new generation of biotherapeutics. In the current study, isolation, phenolic and organic acid composition, and antioxidant, cytotoxic, anticholinesterase activities of truffle Reddellomyces parvulosporus were examined. Four known compounds (brassicasterol (1), ergosterol peroxide (2), fumaric acid (3) and mannitol (4)) were isolated with the combination of chromatographic techniques. Fumaric acid (54.74 +/- 0.85 mu g g(-1)) was found as the major compound by HPLC-DAD. All isolated compounds were bioassayed for antioxidant, cytotoxic, anticholinesterase, anti-tyrosinase, anti-urease, anti-alpha-glucosidase and anti-alpha-amylase activities. Compound 1 indicated notable cytotoxic activity on MCF-7 (IC50: 38.08 +/- 0.75 mu g mL(-1)) and compound 3 on H1299 (IC50: 62.37 +/- 0.75 mu g mL(-1)). Also, compounds 1 (84.55 +/- 1.14%) and 2 (84.90 +/- 0.10%) showed higher anti-urease activity than thiourea (78.57 +/- 0.22%), while compound 2 (66.31 +/- 0.08%) displayed near-standard anti-BChE activity. Also, being the first to emphasise the potential of R. parvulosporus as a natural food additive, this study evidenced its medicinal importance by revealing bioactive compounds and properties.Öğe Amino acid surface modified bioglass: A candidate biomaterial for bone tissue engineering1(Wiley, 2024) Ozkabadayi, Yasin; Turk, Mustafa; Kumandas, Ali; Karahan, SiyamiBioglasses are solid materials consisted of sodium oxide, calcium oxide, silicon dioxide and phosphorus in various proportions and have used in bone tissue engineering. There have been ongoing efforts to improve the surface properties of bioglasses to increase biocompatibility and performance. The aim of the present study is to modify the bioglass surface with an amino acid mixture consisting of arginine, aspartic acid, phenylalanine, cysteine, histidine and lysine, to characterize the surface, and to evaluate the performance and biocompatibility in vitro and in vivo. The untreated bioglass, bioglass kept in simulated body fluid (SBF), and modified bioglass were used in further evaluation. After confirmation of the surface modification with FT-IR analyses and SEM analyses, MC3T3-E1 preosteoblasts adhesion on the surface was also revealed by SEM. The modified bioglass had significantly higher ALP activity in colorimetric measurement, rate of calcium accumulations in Alizarin red s staining, lower rate of cell death in Annexin-V/PI staining to determine apoptosis and necrosis. Having higher cell viability rate in MTT test and absence of genotoxicity in micronucleus test (OECD 487), the modified bioglass was further confirmed for biocompatibility in vitro. The results of the rat tibial defect model revealed that the all bioglass treatments had a significantly better bone healing score compared to the untreated negative control. However, the modified bioglass exhibited significantly better bone healing efforts especially during the first and the second months compared to the other bioglass treatment treatments. As a result, the amino acid surface modification of bioglasses improves the surface biocompatibility and osteogenic performance that makes the amino acid modified bioglass a better candidate for bone tissue engineering. Research Highlights Bioglass surface modification with amino acids contributes to bioglass-tissue interaction with an improved cell attachment. Modified bioglass increases in vitro Alp activity and calcium accumulation, and also positively affects cell behavior by supporting cell adaptation. Bioglass exerts osteogenic potential in vivo especially during early bone healing.Öğe Anti Wnt-1 Monoclonal Antibody's Conjugated with Gold Nanoparticles, Induced Apoptosis on MCF-7 Breast Cancer Cell Lines(Trans Tech Publications Ltd, 2019) Azar, Mehdi Tayybi; Saglam, Necdet; Turk, MustafaWnt-beta Catenin Pathway has an important role in many cancers. Wnt-1 protein from Wnt protein family that regulate this pathway has a special effect on the development of breast cancer. Monoclonal antibodies attach to metal nanoparticles have an important role in diagnosis and treatment of cancers. In this study, Anti Wnt-1 monoclonal antibody was conjugated to the gold nanoparticles synthesized by Turkevich method. Conjugation was achieved using EDC-NHS method. The density of the monoclonal antibodies that bonded to gold nanoparticles was measured by Roche Cobas Integra 400 Plus device. MCF-7 breast cancer cell line was treated with conjugated nanoparticles for 48 h then Double Staining method was used to detect apoptosis cells. The results showed that inhibition of Wnt-1 protein in extracellular matrix causes apoptosis and gold nanoparticles have a positive effect on Anti Wnt-1 monoclonal antibodies and this positive effect causes apoptosis to increase rate in conjugating nanoparticles.Öğe Anti-Epidermal Growth Factor Receptor Aptamer and Antibody Conjugated SPIONs Targeted to Breast Cancer Cells: A Comparative Approach(Amer Scientific Publishers, 2017) Tutkun, Lutfiye; Gunaydin, Esra; Turk, Mustafa; Kutsal, TulinCancer is still one of the major reasons of illness and death throughout the world. Wide range of cancer types occur in many organs like breast, liver, lung, colon etc. The surface characteristics of these cells change, when normal cells transform into cancerous cells. Then, they display unique expression and/or over expression of certain receptors and antigens. Overexpression of these cells compare to normal cells makes them attractive for targeted therapy. Conjugation of monoclonal antibodies and/or small molecules like aptamers to the magnetic nanoparticles is a beneficial method for cancer diagnosis and treatment in terms of targeted therapy. Nanoparticles have a high surface area to volume ratio. Several types of nanoparticles including quantum dots, magnetic iron oxide, gold and polymer based nanoparticles have been developed for cancer applications in the last few years. In this study, developing of an early diagnosis nanoparticular system in order to detect and image human breast cancer cell line of MDA MB 231 was aimed. In this reason, it has been thought to target cell surface receptors of epidermal growth factor which expressed by breast tumor cells of MDA MB 231. To this end, gold coated magnetic nanoparticles were designed and characterized. Average size of synthesized SPIONs and gold-coated SPIONs which were determined by Transmission Electron Microscopy (TEM) were detected as 5.62 +/- 1.49 nm and 57.04 +/- 8.58 nm, respectively. Fluorescein isothiocyanate (FITC)-labelled aptamer and antibody molecules were immobilized onto gold-coated superparamagnetic iron oxide nanoparticles (SPIONs) and then developed carrier system was applied to MDA MB 231 breast cancer cells in the cell culture medium. The estrogen positive human fibroblast cells were chosen as control cells. Proliferation and differentiation of cells were investigated and cytotoxicity of nanoparticular carrier system was examined. The results indicated that various forms and concentrations of SPION were influenced the cytotoxicity results. Moreover, SPIONs were almost nontoxic to both cell lines without any modifications. Apoptosis and necrosis rates were quantified by double-staining of cells. The different forms of SPION formulations applied to MDA MB 231 breast cancer cells resulted in high incidence of apoptosis rather than necrosis. Additionally, the apoptotic and necrotic effects were found to be concentration dependent. Cancer cell selectivity and cell surface receptor based targeting was imaged by the Fluorescent Microscopy imaging technique and quantified by flow cytometry assay. Real time cell analyser (RTCA) was utilized to determine the dynamic cell proliferation on a periodic time intervals. In conclusion, cancer cell surface receptor based targeting by aptamer and monoclonal antibody conjugated SPIONs was successfully achieved.Öğe Antimicrobial, antioxidant, cytotoxic and apoptotic activities of Satureja khuzestanica(Gazi Univ, Fac Med, 2018) Soltanzadeh, Hossein; Acik, Leyla; Turk, Mustafa; Houshmand, Massoud; Shahsavari, GholamrezaObjective: Natural products with antimicrobial effect are currently investigated in order to eliminate the use of synthetic antibiotics that cause the resistance of microorganisms. This study determines the antibacterial, antioxidant activity, and also the cytotoxic and apoptotic effect of Satureja khuzestanica on various cancer cell lines. Materials and methods: The individual constituents were identified by their identical retention indices referring to known compounds from the literature. Methanol and ethanol extracts of S. khuzestanica (25 and 50 mg/(mL)) were screened against four Gram-positive and four Gram-negative bacteria. The plant extracts were tested for their antioxidant activity against 2,2-diphenyl1- picrylhydrazyl (DPPH) radical. The interaction between the extracts and plasmid DNA were analyzed by agarose gel electrophoresis. In addition, the cytotoxic activity of the extracts was evaluated on MCF-7, DLD-1, osteosarcoma, and fibroblast cell line. Finally, gene expression of caspase-3, Bax and Bcl-2 were investigated by real-time PCR. Results: Our result indicated that both extracts showed good inhibitory activity against both Gram-negative (Escherichia coli ATCC 35218) and positive (Bacillus subtilis ATCC 6633) bacteria. In addition, the methanol extract of S. khuzestanica had strong antioxidant activity (IC50= 37.0 +/- 0.3 mu g/mL). The extracts showed a strong effect on plasmid DNA. The methanol extract of S. khuzestanica showed a good concentration-dependent cytotoxicity. Finally, IC50= 47.00a +/- 1.21 mu g/mL, ethanol, and water extract had apoptotic effect in MCF-7 cell line. Discussion: MCF-7 was detected as the most sensitive cell line therefore further studies should be done on this plant extract as a potential anticancer agent against breast cancer.Öğe Antisense oligonucleotide delivery to cancer cell lines for the treatment of different cancer types(Taylor & Francis Ltd, 2016) Kilicay, Ebru; Erdal, Ebru; Hazer, Baki; Turk, Mustafa; Denkbas, Emir BakiAmphiphilic poly(3-hydroxylalkanoate) (PHA) copolymers find interesting applications in drug delivery. The aim of this study was to prepare nucleic acid adsorbed on (PHB-b-PEG-NH2) nanoparticle platform for gene delivery. For this purpose, PHB-b-PEG-NH2 block copolymers were synthesized via transesterification reactions. The copolymers obtained were characterized by Proton Nuclear Magnetic Resonance (H-1-NMR), Fourier Transform Infrared Spectrometer (FTIR), Thermogravimetric Analysis (TGA) and Differential Scanning Calorimetry (DSC) techniques. The cytotoxic, apoptotic and necrotic effects of these nanoparticles in the MDA 231 human breast cancer cell, the A549 human lung cancer cell and the L929 fibroblast cell lines were also investigated.Öğe Apoptotic and necrotic effects of carboxylated quercetin/polyethylenimine complex on HeLa cells(Academic Journals, 2011) Ulug, Murat; Turk, Mustafa; Oguztuzun, Serpil; Menemen, Yusuf; Kahraman, GultenThe effects of quercetin (Q), carboxylated quercetin (CQ) and carboxylated quercetin/polyethylenimine (CQ/PEI) complex on HeLa cell cultures were investigated. Firstly, carboxylated quercetin was acquired through hydroxyl groups of quercetin, using chloroacetic acid. The complex of CQ/PEI was acquired by electron cooperation path over polietilenimine amine groups and quercetin carboxyl groups. CQ and CQ/PEI obtained were characterised by FTIR and H-1-NMR methods. Cytotoxicity was determined by MTT assay. Apoptotic and necrotic indexes were obtained by immunocytochemical staining with the M30 antibodies and double staining and double staining, respectively. It was determined that quercetin caused lower rates of necrosis and apoptosis on HeLa cells by itself, but CQ/PEI complex resulted in high levels. As a result, it was observed that transition of quercetin to HeLa via binding it to polyethylenimine increased its anticarcinogenic effects.Öğe Apoptotic and necrotic effects of plant extracts belonging to the genus Alchemilla L. species on HeLa cells in vitro(Academic Journals, 2011) Turk, Mustafa; Kaya, Bulent; Menemen, Yusuf; Oguztuzun, SerpilApoptotic and necrotic effects of plant extracts belonging to the genus Alchemilla on HeLa cells were investigated. The ratio of viable cells was determined by cell counter. Double staining method for the determination of apoptotic and necrotic index was carried out. The lowest apoptotic effect was found in Alchemilla oriturcica with the ratio of around 14% and the highest apoptotic effect found in Alchemilla trabzonica was 24%. Apoptotic effect was additionally determined by caspase-3 immunostaining. Increasing the concentrations of the extracts, especially in the level of 150 to 200 mu g/mL caused the increase of necrotic effect on cancer cells. Particularly, the effects of A. oriturcica and A. trabzonica extracts with the density of 200 mu g/mL were very high compared to the other species. The flavonoid components were detected in extracts of the species used for this study. The flavonoids identified are orientin (luteolin-8-C-glucoside), hyperoside (quercetin-3-O-galactoside) and isoquercetin (quercetin-3-glucoside) in Alchemilla erythropoda; rutin (quercetin-3-rutinoside), orientin, vitexin (apigenin-8-C-glucoside), hyperoside in Alchemilla ikizdereensis; rutin, hyperoside, isoquercetin (quercetin-3-glucoside) in A. oriturcica, and hyperoside, isoquercetin and quercitrin (quercetin-3-O-rhamnoside) in A. trabzonica.Öğe Aptamer-functionalized magnetic graphene oxide nanocarrier for targeted drug delivery of paclitaxel(Elsevier Science Sa, 2018) Hussien, Nizamudin Awel; Isiklan, Nuran; Turk, MustafaTargeted drug delivery has come out as a golden system owing to its capability to reduce side effects and increase efficacy. The application of graphene oxide (GO) based nanomaterials for targeted delivery has recently attracted several researchers because of their advantageous properties. Herein, we have attempted to prepare aptamer-conjugated magnetic graphene oxide (MGO) nanocarrier, which can specifically target tumor cells. MGO nanocarriers were prepared by attaching Fe3O4 on the layer of GO and then, aptamer (APT) was linked as a targeting moiety. Paclitaxel (PAC), an anti-cancer drug, was also loaded on the nanocarrier. PAC loading and in vitro release results revealed a very good loading performance with entrapment efficiency 95.75% and high pH-responsive release. Cellular toxicity assay showed MGO nanocarriers are biocompatible having cell viability greater than 80% for L-929 fibroblast cell line. Besides, high cytotoxic effect was observed for PAC and PAC loaded MGO (MGO@PAC) on MCF-7 cancer cells, at different drug doses. Furthermore, flow cytometry investigation reveals that the obtained nanocarrier can specifically bind to MCF-7 cancer cells. Therefore, based on the results the prepared superparamagnetic nanocarrier could be considered as a promising agent for cancer drug delivery systems. (C) 2018 Elsevier B.V. All rights reserved.Öğe Autologous stem cell-derived chondrocyte implantation with bio-targeted microspheres for the treatment of osteochondral defects(Bmc, 2019) Bozkurt, Murat; Asik, Mehmet Dogan; Gursoy, Safa; Turk, Mustafa; Karahan, Siyami; Gumuskaya, Berrak; Dogan, MetinBackground Chondral injury is a common problem around the world. Currently, there are several treatment strategies for these types of injuries. The possible complications and problems associated with conventional techniques lead us to investigate a minimally invasive and biotechnological alternative treatment. Combining tissue-engineering and microencapsulation technologies provide new direction for the development of biotechnological solutions. The aim of this study is to develop a minimal invasive tissue-engineering approach, using bio-targeted microspheres including autologous cells, for the treatment of the cartilage lesions. Method In this study, a total of 28 sheeps of Akkaraman breed were randomly assigned to one of the following groups: control (group 1), microfracture (group 2), scaffold (group 3), and microsphere (group 4). Microspheres and scaffold group animals underwent adipose tissue collection prior to the treatment surgery. Mesenchymal cells collected from adipose tissue were differentiated into chondrocytes and encapsulated with scaffolds and microspheres. Osteochondral damage was conducted in the right knee joint of the sheep to create an animal model and all animals treated according to study groups. Results Both macroscopic and radiologic examination showed that groups 3 and 4 have resulted better compared to the control and microfracture groups. Moreover, histologic assessments indicate hyaline-like cartilage formations in groups 3 and 4. Conclusion In conclusion, we believe that the bio-targeted microspheres can be a more effective, easier, and safer approach for cartilage tissue engineering compared to previous alternatives.Öğe Bi-layered constructs of poly(glycerol-sebacate)-beta-tricalcium phosphate for bone-soft tissue interface applications(Elsevier Science Bv, 2017) Tevlek, Atakan; Hosseinian, Pezhman; Ogutcu, Cansel; Turk, Mustafa; Aydin, Halil MuratThis study aims to establish a facile protocol for the preparation of a bi-layered poly(glycerol-sebacate) (PGS)/beta-tricalcium phosphate (beta-TCP) construct and to investigate its potential for bone-soft tissue engineering applications. The layered structure was prepared by distributing the ceramic particles within a prepolymer synthesized in a microwave reactor followed by a cross-linking of the final construct in vacuum (<10 mbar). The vacuum stage led to the separation of cross-linked elastomer (top) and ceramic (bottom) phases. Results showed that addition of beta-TCP particles to the elastomer matrix after the polymerization led to an increase in compression strength (up to 14 +/- 2.3 MPa). Tensile strength (sigma), Young's modulus (E), and elongation at break (%) values were calculated as 0.29 +/- 0.03 MPa and 0.21 +/- 0.03; 038 +/- 0.02 and 1.95 +/- 0.4; and 240 +/- 50% and 24 +/- 2% for PGS and PGS/beta-TCP bi-layered constructs, respectively. Morphology was characterized by using Scanning Electron Microscopy (SEM) and micro-computed tomography (mu-CT). Tomography data revealed an open porosity of 35% for the construct, mostly contributed from the ceramic phase since the elastomer side has no pore. Homogeneous beta-TCP distribution within the elastomeric structure was observed. Cell culture studies confirmed biocompatibility with poor elastomer-side and good bone-side cell attachment. In a further study to investigate the osteogenic properties, the construct were loaded with BMP-2 and/or TGF-beta 1. The PGS/beta-TCP bi-layered constructs with improved mechanical and biological properties have the potential to be used in bone-soft tissue interface applications where soft tissue penetration is a problem. (C) 2016 Elsevier B.V. All rights reserved.Öğe Biocompatible and biodegradable poly(Tannic Acid) hydrogel with antimicrobial and antioxidant properties(Elsevier, 2016) Sahiner, Nurettin; Sagbas, Selin; Sahiner, Mehtap; Silan, Coskun; Aktas, Nahit; Turk, MustafaA novel resourceful bulk poly(Tannic Acid) (p(TA)) hydrogel was prepared by crosslinking TA molecules with an epoxy crosslinker, trimethylolpropane triglycidyl ether (TMPGDE), in an autoclave at 90 degrees C for 2 h. The obtained p(TA) hydrogels were in disk form and have highly porous morphology. The swelling characteristics of p(TA) hydrogels were investigated in wound healing pH conditions of pH 5.4, 7.4, and 9 at 37.5 degrees C, and the hydrogels showed good swelling and moisture content behavior. Especially, p(TA) hydrogels were found to be sensitive to pH 9 with 1669% maximum swelling. P(TA) hydrogels were completely degraded at pH 9 hydrolytically in 9 days. Total phenol contents and the effects of scavenging ABTS radicals of degraded p(TA) hydrogels at pH 5.4, 7.4, and 9 were evaluated and calculated in terms of gallic acid equivalent and trolox equivalent antioxidant capacity, respectively, and found to be very effective. Moreover, degraded p(TA) hydrogels display strong antimicrobial behavior against gram positive Staphylococcus aureus, Bacillus subtilis, gram negative Pseudomonas aeruginosa bacteria strains and Candida albicans fungus strain. The WST-1 results indicated that bulk p(TA) hydrogels have no cyctotoxicity to the L929 fibroblast cell line in vitro. (C) 2015 Elsevier B.V. All rights reserved.Öğe Bioengineering functional copolymers. XV. Synthesis of organoboron amide-ester branched derivatives of oligo(maleic anhydride) and their interaction with HeLa and L929 fibroblast cells(Inst Organic Chem And Biochem, 2011) Kahraman, Gulten; Turk, Mustafa; Rzayev, Zakir M. O.; Unsal, M. Elif; Soylemez, ErnurNovel bioengineering functional organoboron oligomers were synthesized by (i) amidolysis of oligo(maleic anhydride) (OMA) with 2-aminoethyldiphenylborinate (2-AEPB), (ii) esterification of organoboron oligomer (OMA-B) with alpha-hydroxy-omega-methoxypoly(ethylene oxide) (PEO) as a compatibilizer and (iii) conjugation of organoboron PEO branches (OMA-B-PEO) with folic acid as a taggering agent. Structure and composition of the synthesized oligomers were characterized by FTIR-ART and (1)H ((13)C) NMR spectroscopy, chemical and physical analysis methods. Interaction of functional oligomers and oligomer center dot center dot center dot FA complex (OMA-B-PEO-F) with HeLa and L929 fibroblast cells were investigated by using different biochemical methods such as cytotoxicity, statistical, apoptotic and necrotic cell indexes, double staining and caspase-3 immunostaining, light and fluorescence inverted microscope analyses. It was found that citotoxisity and apoptotic/necrotic effects of oligomers significantly depend on the structure and composition of studied oligomers, and increase the following raw: OMA << OMA-B < OMA-B-PEO < OMA-B-PEO-F. A folic acid complex (MA-PEG-B-F) at 400 mu g ml(-1) (2.36 mu mol ml(-1)) concentration as a therapeutic drug exhibits minimal toxcisity toward the fibroblast cells, but influential for HeLa cells.Öğe Calcified and mechanically debilitated three-dimensional hydrogel environment induces hypertrophic trend in chondrocytes(Sage Publications Ltd, 2016) Celik, Ekin; Bayram, Cem; Akcapinar, Rumeysa; Turk, Mustafa; Denkbas, Emir BakiCurrently, the main focus on tissue engineering strategies is to mimic the extracellular matrix of the related tissues. Many studies accomplished to build tissue scaffolds to act as the natural surroundings of the specific interest, which can be established to behave like either healthy or unhealthy tissues. The latter one of these conditions is a quite new approach and crucial for the design of three-dimensional in vitro disease models. This study investigates the potential of a composite scaffold consisting hydroxyapatite-integrated fluorenyl-9-methoxycarbonyl diphenylalanine hydrogels by focusing on the optimization of this hybrid scaffold for the development of an in vitro model of degenerative cartilage. Cell growth, chondrocyte proliferation, extracellular matrix production, hypertrophy marker monitoring, scaffold mechanical properties, and morphological analysis were evaluated. Fluorenyl-9-methoxycarbonyl diphenylalanine dipeptides were dissolved in null cell culture media and pH decreased sequentially to compel peptides to self-organize into fibrous hydrogel scaffolds. Nano-hydroxyapatite crystals were incorporated into fluorenyl-9-methoxycarbonyl diphenylalanine hydrogels during the gelation to investigate the effect on chondrocytes. It is observed that hydroxyapatite incorporation into peptide hydrogels significantly increased the alkaline phosphatase activity and assymetrical cell divisions, which is appraised as an outcome of chondrocyte hypertrophy. It is concluded that chondrocytes develop a hypertrophic potential when they are cultured in a media with nano-hydroxyapatites in a three-dimensional cell culture matrix mimicking the extracellular matrix conditions of degenerative cartilage.Öğe Chalcone-based dipolar cycloaddition of novel heteroaromatic compounds: Their anticancer examination(Elsevier, 2023) Kinali, Mehmet; Col, Sumeyye; Coban, Canan Cakir; Turk, Mustafa; Aydin, Gokay; Emirik, Mustafa; Baran, ArifIn this study, new chalcone-isoxazole-based hybrid derivatives (12, 13, 18, and 21) and chalcone-triazole hybrid molecules (26) were synthesized using the click chemistry approach. Among these hybrid molecules, 12, 13, 21, and 26 were obtained by combining azole pharmacophore groups such as thiazole, isoxazole, and 1,2,3-triazole. In addition, a new bioactive hybrid molecule (E)-3-(2-(1-((4- (3-(4-((3-(4-(benzyloxy) phenyl) isoxazol-5-yl) methoxy) phenyl)-3-oxoprop-1-en-1-yl) phenoxy)methyl)-1H-1,2,3-triazol-4-yl)thiazol-4-yl)-2H-chromen-2one (18), which is a chalcone-isoxazole hybrid derivative with azido thiazole coumarin substitution, was synthesized. The chemical structures of synthesized compounds were characterized by spectroscopic techniques such as 1H NMR, 13C NMR, FT-IR, and MALDI-TOF MS (18, 21, and 26). These novel chalcone-azole hybrids (12, 13, 18, 21, and 26) were investigated for their in-vitro anticancer activity against A549 cells, Capan-1 cell lines, and a healthy L929 fibroblast cell line, as well as the apoptotic and necrotic effects of these compounds on Capan1 cell lines. Among the compounds tested, hybrid 18 showed the best activity in the A549 cell line, while it showed moderate activity in the Capan-1 cell line.Öğe Characterization of chondrocytes cultured on catechin-loaded alginate-chitosan scaffolds(Informa Healthcare, 2013) Turk, Mustafa; Karahan, Siyami; Cinar, Miyase; Kucuk, Sebnem; Dincel, Gungor CagdasBovine chondrocytes were seeded into scaffolds of a high molecular weight chitosan and alginate with a pore size of 50-350 mu m with or without catechin. In polymerase chain reaction (PCR), unlike type II, collagen type I was no longer expressed at day 14. The DNA content increased until day 8 and began declining, indicating cell detachment. The GAG content increased during the first 12 days. The percentage of round and collagen type II immunoreactive cells increased over the time. Catechin has some protective properties on chondrocytes seeded on the alginate-chitosan scaffolds during the first 12 days by means of DNA and chondrocyte morphology (p < 0.05).Öğe Characterization of p(PmO), p(LO) and p(RO) organoparticles, their bioactivity properties and their effect on pancreatic cancer Capan-1 cell(Elsevier Science Sa, 2023) Alpaslan, Duygu; Turan, Abdullah; Dudu, Tuba Ersen; Bozer, Busra Moran; Aktas, Nahit; Turk, MustafaFor the first time in the literature, p (PmO), p (LO) and p (RO) organo-particles were synthesized from Peppermint oil, Lemon oil and Rose oil. Of the organo-particles L-929 cell line viability/cytotoxicity and anticancer effect against Capan-1 pancreatic cancer cell line were investigated. p (PmO), p (LO) and p (RO) organoparticles were featured by thermogravimetry (TGA), Fourier Transform Infrared Spectroscopy (FTIR), Scanning electron microscope (SEM), Particle size (DLS), and particle charge (zeta potential, Zeta) analyses. Antioxidant, biocompatible, and antimicrobial activities and in vitro cytotoxicity specialties were investigated. In studies on Capan-1 and L-929 cell lines, it was observed that p (PmO), p (LO) and p (RO) organo-particles were effective on L-929 fibroblast cell line on Capan-1 pancreatic cancer cell line. In addition, it was observed that p (PmO), p (LO) and p (RO) organo-particles were not toxic in L-929 cell lines at high doses. When the Capan-1 cell line MTT analysis results of p (PmO), p (LO) and p (RO) organo-particles were examined, a difference was observed between cell viability rates and apoptosis and necrosis values. The highest % apoptosis rate was observed in the p (RO) organo particle.Öğe Chemical constituents and their bioactivities from truffle Hysterangium inflatum(Springer, 2021) Tas, Meltem; Kucukaydin, Selcuk; Tel-Cayan, Gulsen; Duru, Mehmet Emin; Ozturk, Mehmet; Turk, MustafaHysterangium inflatum is a truffle that grows naturally in the roots of Eucalyptus sp. and is distributed along the Mediterranean and Aegean coasts. Chemical investigation of the H. inflatum enabled to isolate of a new cerebroside, hysteroside (1), and seven known compounds namely, psyllic acid (2), brassicasterol (3), ergosterol (4), ergosterol d (5), ergosterol peroxide (6), ergosta-7,9,22-triene-3-O-beta-d-glucoside (7) and mannitol (8). IR, NMR, MS techniques were used for structural elucidation and supported with literature data. Antioxidant, cholinesterase, urease, and tyrosinase inhibitory and cytotoxic activities on MCF-7 breast, H-1299 lung cancer cells, and murine fibroblast (L929) non-cancerous cells of extracts and isolated compounds from H. inflatum were analyzed. All the isolated compounds, except compounds 2 and 8, displayed considerable cytotoxic activities against MCF-7 and H-1299 cancer cells. Compounds 1 (IC50: 18.11 mu g/mL) and 5 (IC50: 24.93 mu g/mL) were the most effective against MCF-7, while compounds 6 (IC50: 27.61 mu g/mL), 1 (IC50: 36.20 mu g/mL) and 5 (IC50: 38.62 mu g/mL) showed most potent toxicity against H-1299 and the extracts and compounds have no toxic effect on L929. Among the extracts, the methanol extract displayed the best antioxidant activity in all assays. Compound 1 exhibited highest enzyme inhibition activities with value of 58.71%, 52.84%, 45.37%, and 35.63%, against urease, tyrosinase, butrylcholinesterase (BChE), and acetylcholinesterase (AChE) enzymes at 100 mu g/mL concentration, among the isolated compounds. These results support that H. inflatum is a steroid-rich truffle and might be a potential source, especially for ergostane type steroids.Öğe Cytotoxic, apoptotic and necrotic effects of starch coated copper nanoparticles on Capan 1 pancreatic cancer cells(Elsevier, 2023) Ilbasmis-Tamer, Sibel; Turk, Mustafa; Evran, Sefika; Boyaci, Ismail Hakki; Ciftci, Hakan; Tamer, UgurIn the present study we utilized a green synthesis approach by reduction route with ascorbic acid in the presence of starch as the capping agent. Here we aimed to generate a stable starch coating copper nanoparticles (Cu-SNPs) and to investigate their apoptotic and necrotic effects on Capan 1 pancreatic cancer cell line. The water soluble monodisperse starch-protected zero-valent copper nanoparticles were characterized by transmission electron microscopy, XPS, FTIR analysis. TEM images showed that the presence of metallic copper nanoparticles with anisotrophic morphologies and the sizes of 90% copper nanoparticles were in 50 nm and 80 nm range. Cetyl-trimethylammonium bromide (CTAB) coated copper nanoparticles was also synthesized to compare the stability of starch coating copper nanoparticles. It was also found that copper nanoparticles were significantly protected by starch coating. Cu-SNPs exhibited stronger antimicrobial effects on Staphylocossus and Enterococcus. In vitro cell culture studies demonstrated that Cu-SNPs were not cytotoxic on L929-fibroblasts and Capan 1 pancreatic cancer cell in all concentrations used here (6.25-100 ppm) in different extends. The cytotoxic, necrotic, and apoptotic effects of Cu-SNPs on the Capan 1 pancreatic cancer cell line have been exhibited. Furthermore, the micronucleus test and hemolytic activity test were also performed.Öğe Cytotoxicity and apoptotic effects of nickel oxide nanoparticles in cultured HeLa cells(Via Medica, 2010) Ada, Kezban; Turk, Mustafa; Oguztuzun, Serpil; Kilic, Murat; Demirel, Mehmet; Tandogan, Nisa; Latif, OzturkThe aim of this study was to observe the cytotoxicity and apoptotic effects of nickel oxide nanoparticles on human cervix epithelioid carcinoma cell line (HeLa). Nickel oxide precursors were synthesized by an nickel sulphate-excess urea reaction in boiling aqueous solution. The synthesized NiO nanoparticles (<200 nm) were investigated by X-ray diffraction analysis and transmission electron microscopy techniques. For cytotoxicity experiments, HeLa cells were incubated in 50-500 mu g/mL NiO for 2, 6, 12 and 16 hours. The viable cells were counted with a haemacytometer using light microscopy. The cytotoxicity was observed low in 50-200 mu g/mL concentration for 16 h, but high in 400-500 mu g/mL concentration for 2-6 h. HeLa cells' cytoplasm membrane was lysed and detached from the well surface in 400 mu g/mL concentration NiO nanoparticles. Double staining and M30 immunostaining were performed to quantify the number of apoptotic cells in culture on the basis of apoptotic cell nuclei scores. The apoptotic effect was observed 20% for 16 h incubation.
