Apoptotic and necrotic effects of carboxylated quercetin/polyethylenimine complex on HeLa cells
Yükleniyor...
Tarih
2011
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Academic Journals
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The effects of quercetin (Q), carboxylated quercetin (CQ) and carboxylated quercetin/polyethylenimine (CQ/PEI) complex on HeLa cell cultures were investigated. Firstly, carboxylated quercetin was acquired through hydroxyl groups of quercetin, using chloroacetic acid. The complex of CQ/PEI was acquired by electron cooperation path over polietilenimine amine groups and quercetin carboxyl groups. CQ and CQ/PEI obtained were characterised by FTIR and H-1-NMR methods. Cytotoxicity was determined by MTT assay. Apoptotic and necrotic indexes were obtained by immunocytochemical staining with the M30 antibodies and double staining and double staining, respectively. It was determined that quercetin caused lower rates of necrosis and apoptosis on HeLa cells by itself, but CQ/PEI complex resulted in high levels. As a result, it was observed that transition of quercetin to HeLa via binding it to polyethylenimine increased its anticarcinogenic effects.
Açıklama
Anahtar Kelimeler
Quercetin, HeLa, cancer, apoptosis, necrosis, cytotoxicity
Kaynak
African Journal Of Pharmacy And Pharmacology
WoS Q Değeri
Q4
Scopus Q Değeri
N/A
Cilt
5
Sayı
7
Künye
closedAccess
