Membrane with incorporated hydrophobic ligand for hydrophobic interaction with proteins: application to lipase adsorption
Yükleniyor...
Tarih
2002
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, phenylalanine as a hydrophobic ligand was covalently attached on a comonomer, methacryloyl chloride. Then, poly(2-hydroxyethyl methacrylate-co-methacrylamido-phenylalanine), poly(HEMA/MAPA), membranes were prepared by UV-initiated photopolymerization of 2-hydroxyethyl methacrylate and methacrylamido-phenylalanine. The lipase adsorption of these poly(HEMA/MAPA) membranes was determined by changing the hydrophobic ligand density, pH, temperature and concentration of lipase in the adsorption medium. The lipase adsorption capacity of the membranes increased as the ligand density on the membrane surface increased. The nonspecific adsorption of lipase on the poly(2-hydroxyethyl methacrylate) membranes was negligible (12 mug cm(-2) of membrane). The adsorption phenomena appeared to follow a typical Langmuir isotherm. The maximum adsorption capacity (Q(m)) of the poly(HEMA/MAPA-5) membrane for lipase was 215 mug cm(-2) of membrane. The equilibrium constant (k(d)) value was 1.43mg ml(-1). The lipase could repeatedly be adsorbed and desorbed on the affinity membrane without any significant loss in the adsorption capacity of the membrane. (C) 2002 Society of Chemical Industry.
Açıklama
Conference on Polymers in the 3rd Millennium -- SEP 02-06, 2001 -- MONTPELLIER, FRANCE
Anahtar Kelimeler
affinity membrane, hydrophobic ligand, phenylalanine, adsorption, protein separation, lipase
Kaynak
Polymer International
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
51
Sayı
10
Künye
closedAccess
