Membrane with incorporated hydrophobic ligand for hydrophobic interaction with proteins: application to lipase adsorption

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dc.contributor.authorBayramoglu, G.
dc.contributor.authorDenizli, A.
dc.contributor.authorArica, M.Y.
dc.date.accessioned2020-06-25T17:34:59Z
dc.date.available2020-06-25T17:34:59Z
dc.date.issued2002
dc.departmentKırıkkale Üniversitesi
dc.descriptionConference on Polymers in the 3rd Millennium -- SEP 02-06, 2001 -- MONTPELLIER, FRANCE
dc.description.abstractIn this study, phenylalanine as a hydrophobic ligand was covalently attached on a comonomer, methacryloyl chloride. Then, poly(2-hydroxyethyl methacrylate-co-methacrylamido-phenylalanine), poly(HEMA/MAPA), membranes were prepared by UV-initiated photopolymerization of 2-hydroxyethyl methacrylate and methacrylamido-phenylalanine. The lipase adsorption of these poly(HEMA/MAPA) membranes was determined by changing the hydrophobic ligand density, pH, temperature and concentration of lipase in the adsorption medium. The lipase adsorption capacity of the membranes increased as the ligand density on the membrane surface increased. The nonspecific adsorption of lipase on the poly(2-hydroxyethyl methacrylate) membranes was negligible (12 mug cm(-2) of membrane). The adsorption phenomena appeared to follow a typical Langmuir isotherm. The maximum adsorption capacity (Q(m)) of the poly(HEMA/MAPA-5) membrane for lipase was 215 mug cm(-2) of membrane. The equilibrium constant (k(d)) value was 1.43mg ml(-1). The lipase could repeatedly be adsorbed and desorbed on the affinity membrane without any significant loss in the adsorption capacity of the membrane. (C) 2002 Society of Chemical Industry.en_US
dc.description.sponsorshipSoc Chem Ind & Polymer Int, IEEE Dielectr & Electr Insulat Soc, IAE, SISM, WILEY, IEEE, DEISen_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/pi.899
dc.identifier.endpage972en_US
dc.identifier.issn0959-8103
dc.identifier.issn1097-0126
dc.identifier.issue10en_US
dc.identifier.scopus2-s2.0-0036809489
dc.identifier.scopusqualityQ1
dc.identifier.startpage966en_US
dc.identifier.urihttps://doi.org/10.1002/pi.899
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2977
dc.identifier.volume51en_US
dc.identifier.wosWOS:000179103200020
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWileyen_US
dc.relation.ispartofPolymer International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectaffinity membraneen_US
dc.subjecthydrophobic liganden_US
dc.subjectphenylalanineen_US
dc.subjectadsorptionen_US
dc.subjectprotein separationen_US
dc.subjectlipaseen_US
dc.titleMembrane with incorporated hydrophobic ligand for hydrophobic interaction with proteins: application to lipase adsorptionen_US
dc.typeArticle

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