Smart and cationic poly(NIPA)/PEI block copolymers as non-viral vectors: in vitro and in vivo transfection studies

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dc.contributor.authorTürk, M.
dc.contributor.authorDinçer, S.
dc.contributor.authorPiskin, E.
dc.date.accessioned2020-06-25T17:43:38Z
dc.date.available2020-06-25T17:43:38Z
dc.date.issued2007
dc.description.abstractIn this study, in vitro and in vivo transfection of temperature-sensitive, polycationic poly(N-isopropylacrylamide) and polyethyleneimine copolymers (poly(NIPA)/PE125L) were performed. Copolymer and copolymer-plasmid DNA (pDNA) complexes were positively charged as +7.6 and +12.8, respectively. Gel retardation assay confirmed good complex formation and release of plasmid DNA in response to temperature and pH. Cytotoxicity tests showed at least 80% smooth muscle cell (SMC) viability. The uptake of the complexes by SMCs was quite high; however, the best gene expression efficiency achieved with the copolymeric vectors was about 30% with the complex prepared with a polymer: plasmid ratio of 6. Gene expression efficiency was enhanced up to 50% by changing the temperature from 37 degrees C to 28 degrees C. Preliminary in vivo studies were performed above and below lower critical solution temperature (LCST) in lung, heart, liver, kidney, muscle and also subcutaneously in 5 week-old mice. The gene expression ratio was higher in lung, tibial muscle and subcutaneously than in other tissues (heart, liver and kidney) above LCST. Then, temperature decrease caused an increase in the amount of gene expression in tibial muscle and subcutaneously, revealing the contribution of temperature-sensitivity on DNA release and gene expression. Copyright (c) 2007 John Wiley & Sons, Ltd.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1002/term.47
dc.identifier.endpage388en_US
dc.identifier.issn1932-6254
dc.identifier.issue5en_US
dc.identifier.pmid18038432
dc.identifier.scopus2-s2.0-39649124061
dc.identifier.scopusqualityQ1
dc.identifier.startpage377en_US
dc.identifier.urihttps://doi.org10.1002/term.47
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3839
dc.identifier.volume1en_US
dc.identifier.wosWOS:000256520100006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofJournal Of Tissue Engineering And Regenerative Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectgene therapyen_US
dc.subjectnon-viral vectorsen_US
dc.subjectpoly(NIPA)-PEI copolymeren_US
dc.subjecttemperature-sensitive polymeren_US
dc.subjectin vitro DNA uptake and gene expressionen_US
dc.subjectprimary smooth muscle cellsen_US
dc.subjectin vivo GFP expressionen_US
dc.titleSmart and cationic poly(NIPA)/PEI block copolymers as non-viral vectors: in vitro and in vivo transfection studiesen_US
dc.typeArticle

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