Assessment of free-radical scavenging activity of Gypsophila pilulifera: assay-guided isolation of verbascoside as the main active component
Yükleniyor...
Tarih
2014
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Soc Brasileira Farmacognosia
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Gypsophila pilulifera, Boiss & Heldr, Caryophyllaceae, is a perennial medicinal herb that grows in the-southwestern region of Turkey. Except for only one report on the isolation-of cytotoxic. saponins from the underground parts of G. pilulifera, there are no published thorough phytochemical or bioactivity studies on this specie. In the present study; the free-radical Scavenging activity of extracts and fractions of the-stems of G. pilulifera was evaluated, using a. slightly modified and more-precise-version of the 2,2-diphenyl-1-picrylhydrazyl (DRPH) assay, :reported here for the first time. The DPPH assay-guided HPLC-PDA-purification of the active solid-phase extraction fraction (50% methanol in water) of the methanolic-extract exhibited verbascoside as the main free-radical Scavenger present in this species. The structure of this active compound was resolved by spectroscopy, and the free-radical scavenging potential of verbascoside was determined. (C) 2014 Sociedade Brasileira. de Farmacognosia. Published by Elsevier Editora Ltda. All rights reserved..
Açıklama
Anahtar Kelimeler
Gypsophila pilulifera, Free-radical scavenger, Solid-phase extraction, High-performance liquid chromatography (HPLC), Phenylethanoid, Verbascoside
Kaynak
Revista Brasileira De Farmacognosia-Brazilian Journal Of Pharmacognosy
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
24
Sayı
1
Künye
Chima, N.K., Nahar, L., Majinda, R.R., Çelik, S., & Sarker, S.D. (2014). Assessment of free-radical scavenging activity of Gypsophila pilulifera: assay-guided isolation of verbascoside as the main active component. Revista Brasileira De Farmacognosia-brazilian Journal of Pharmacognosy, 24(1), 38-43.
