Preparation of reversibly immobilized lysozyme onto Procion Green H-E4BD-attached poly(hydroxyethylmethacrylate) film for hydrolysis of bacterial cells
Yükleniyor...
Tarih
2001
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Sci Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The flat sheet support for enzyme immobilization was prepared by UV-initiated photopolymerization of 2-hydroxyethyl-methacrylate (HEMA) in the presence of an initiator (alpha alpha'-azoisobutyronitrile. AIBN). An affinity dye, Procion Green H-E4BD, was attached covalently under alkaline conditions and the pHEMA-Procion Green H-E4BD-attached film was used for the immobilization of lysozyme via adsorption. The amount of attached dye on the pHEMA film was 160 mu mol m(2) and the water content of the dye-attached pHEMA film was 69%. The lysozyme adsorption capacity of the dye-attached pHEMA film was determined under conditions of different pH and with different initial concentrations of enzyme in the medium. The maximum lysozyme adsorption capacity of the dye-attached pHEMA film, under the specified experimental conditions was 3.92 g m(-2). Non-specific adsorption of the lyzozyme on the pHEMA film was negligible, Optimum reaction pH was 6.0 for the free and 7.0 for adsorbed enzyme. The free enzyme had an optimum temperature of 35 degreesC. whereas it shifted to 40 degreesC for the immobilized enzyme system. The enzyme could be repeatedly adsorbed and desorbed from the dye-attached pHEMA film without any significant loss in adsorption capacity. (C) 2001 Elsevier Science Ltd. All rights reserved.
Açıklama
Anahtar Kelimeler
lysozyme, Procion Green H-E4BD, pHEMA affinity film, immobilization, adsorption
Kaynak
Food Chemistry
WoS Q Değeri
Q1
Scopus Q Değeri
Q1
Cilt
75
Sayı
3
Künye
closedAccess
