Preparation of reversibly immobilized lysozyme onto Procion Green H-E4BD-attached poly(hydroxyethylmethacrylate) film for hydrolysis of bacterial cells

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dc.contributor.authorKacar, Y
dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:34:47Z
dc.date.available2020-06-25T17:34:47Z
dc.date.issued2001
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe flat sheet support for enzyme immobilization was prepared by UV-initiated photopolymerization of 2-hydroxyethyl-methacrylate (HEMA) in the presence of an initiator (alpha alpha'-azoisobutyronitrile. AIBN). An affinity dye, Procion Green H-E4BD, was attached covalently under alkaline conditions and the pHEMA-Procion Green H-E4BD-attached film was used for the immobilization of lysozyme via adsorption. The amount of attached dye on the pHEMA film was 160 mu mol m(2) and the water content of the dye-attached pHEMA film was 69%. The lysozyme adsorption capacity of the dye-attached pHEMA film was determined under conditions of different pH and with different initial concentrations of enzyme in the medium. The maximum lysozyme adsorption capacity of the dye-attached pHEMA film, under the specified experimental conditions was 3.92 g m(-2). Non-specific adsorption of the lyzozyme on the pHEMA film was negligible, Optimum reaction pH was 6.0 for the free and 7.0 for adsorbed enzyme. The free enzyme had an optimum temperature of 35 degreesC. whereas it shifted to 40 degreesC for the immobilized enzyme system. The enzyme could be repeatedly adsorbed and desorbed from the dye-attached pHEMA film without any significant loss in adsorption capacity. (C) 2001 Elsevier Science Ltd. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0308-8146(01)00208-4
dc.identifier.endpage332en_US
dc.identifier.issn0308-8146
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0034765419
dc.identifier.scopusqualityQ1
dc.identifier.startpage325en_US
dc.identifier.urihttps://doi.org/10.1016/S0308-8146(01)00208-4
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2887
dc.identifier.volume75en_US
dc.identifier.wosWOS:000172349500009
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofFood Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectlysozymeen_US
dc.subjectProcion Green H-E4BDen_US
dc.subjectpHEMA affinity filmen_US
dc.subjectimmobilizationen_US
dc.subjectadsorptionen_US
dc.titlePreparation of reversibly immobilized lysozyme onto Procion Green H-E4BD-attached poly(hydroxyethylmethacrylate) film for hydrolysis of bacterial cellsen_US
dc.typeArticle

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