Neuroprotective effects of thymoquinone against spinal cord ischemia-reperfusion injury by attenuation of inflammation, oxidative stress, and apoptosis

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dc.contributor.authorGokce, Emre Cemal
dc.contributor.authorKahveci, Ramazan
dc.contributor.authorGokce, Aysun
dc.contributor.authorCemil, Berker
dc.contributor.authorAksoy, Nurkan
dc.contributor.authorSargon, Mustafa Fevzi
dc.contributor.authorKahveci, Ozan
dc.date.accessioned2020-06-25T18:16:30Z
dc.date.available2020-06-25T18:16:30Z
dc.date.issued2016
dc.departmentKırıkkale Üniversitesi
dc.descriptionKISA, Ucler/0000-0002-8131-6810
dc.description.abstractOBJECTIVE lschemia-reperfusion (I/R) injury of the spinal cord following thoracoabdominal aortic surgery remains the most devastating complication, with a life-changing impact on the patient. Thymoquinone (TQ), the main constituent of the volatile oil from Nigella sativa seeds, is reported to possess strong antioxidant, antiinflammatory, and antiapoptotic properties. This study investigated the effects of TQ administration following I/R injury to the spinal cord. METHODS Thirty-two rats were randomly allocated into 4 groups. Group 1 underwent only laparotomy. For Group 2, aortic clip occlusion was introduced to produce I/R injury. Group 3 was given 30 mg/kg of methylprednisolone intraperitoneally immediately after the I/R injury. Group 4 was given 10 mg/kg of TQ intraperitoneally for 7 days before induction of spinal cord I/R injury, and administration was continued until the animal was euthanized. Locomotor function (Basso, Beattie, and Bresnahan scale and inclined plane test) was assessed at 24 hours postischemia. Spinal cord tissue samples were harvested to analyze tissue concentrations of malondialdehyde, nitric oxide, tumor necrosis factor-alpha, interleukin-1, superoxide dismutase, glutathione-peroxidase, catalase, and caspase-3. In addition, histological and ultrastructural evaluations were performed. RESULTS Thymoquinone treatment improved neurological outcome, which was supported by decreased levels of oxidative products (malondialdehyde and nitric oxide) and proinflammatory cytokines (tumor necrosis factor-alpha and interleukin-1), increased activities of antioxidant enzymes (superoxide dismutase, glutathione-peroxidase, and catalase), as well as reduction of motor neuron apoptosis. Light microscopy and electron microscopy results also showed preservation of tissue structure in the treatment group. CONCLUSIONS As shown by functional, biochemical, histological, and ultrastructural analysis, TQ exhibits an important protective effect against I/R injury of the spinal cord.en_US
dc.identifier.citationGökce, E. C., Kahveci, R., Gökce, A., Cemil, B., Aksoy, N., Sargon, M. F., Kısa, Ü., Erdoğan, B., Güvenç, Y., Alagöz, F., & Kahveci, O. (2016). Neuroprotective effects of thymoquinone against spinal cord ischemia-reperfusion injury by attenuation of inflammation, oxidative stress, and apoptosis. Journal of neurosurgery. Spine, 24(6), 949–959.en_US
dc.identifier.doi10.3171/2015.10.SPINE15612
dc.identifier.endpage959en_US
dc.identifier.issn1547-5654
dc.identifier.issn1547-5646
dc.identifier.issue6en_US
dc.identifier.pmid26871652
dc.identifier.scopus2-s2.0-85020261227
dc.identifier.scopusqualityQ1
dc.identifier.startpage949en_US
dc.identifier.urihttps://doi.org/10.3171/2015.10.SPINE15612
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6550
dc.identifier.volume24en_US
dc.identifier.wosWOS:000376476100013
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAmer Assoc Neurological Surgeonsen_US
dc.relation.ispartofJournal Of Neurosurgery-Spine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectthymoquinoneen_US
dc.subjectmethylprednisoloneen_US
dc.subjectneuroprotectionen_US
dc.subjectspinal cord ischemia-reperfusion injuryen_US
dc.subjectoxidative stressen_US
dc.subjectinflammationen_US
dc.subjectapoptosisen_US
dc.subjectspinal cord injuryen_US
dc.subjectraten_US
dc.titleNeuroprotective effects of thymoquinone against spinal cord ischemia-reperfusion injury by attenuation of inflammation, oxidative stress, and apoptosisen_US
dc.typeArticle

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