Potential mechanistic pathways underlying intestinal and hepatic effects of kefir in high-fructose-fed rats

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dc.authoridAkar, Fatma/0000-0002-5432-0304
dc.authoridBostanci, Aykut/0000-0002-6855-0645
dc.authoridSadi, Gokhan/0000-0002-6422-1203
dc.authoridSumlu, Esra/0000-0002-5004-5958
dc.contributor.authorAkar, Fatma
dc.contributor.authorSumlu, Esra
dc.contributor.authorAlcigir, Mehmet Eray
dc.contributor.authorBostanci, Aykut
dc.contributor.authorSadi, Gokhan
dc.date.accessioned2025-01-21T16:43:33Z
dc.date.available2025-01-21T16:43:33Z
dc.date.issued2021
dc.departmentKırıkkale Üniversitesi
dc.description.abstractExcess intake of fructose may contribute to the high prevalence of metabolic disorder. In this study, we investigated the effects of kefir supplementation on the intestine-liver-adipose tissue axis in metabolic disorder induced by high-fructose diet in rats to describe mechanistic action and potential therapeutic value of kefir. Fructose was given to the rats as a 20% solution in drinking water for 15 weeks. Kefir was administrated by gastric gavage once a day during the final six weeks. Kefir supplementation improved metabolic parameters, including plasma triglyceride and insulin levels; hepatic weight, triglyceride content and fatty degeneration; omental fat mass in fructose-fed rats. Kefir supplementation decreased the ratio of Firmicutes/Bacteroidetes in feces, as well as necrotic degeneration, expression levels of nuclear factor-kappa B (NF-kappa B), and inducible nitric oxide synthase (iNOS), but increased expression of tight-junction proteins occludin and claudin-1, in the ileum of the fructose-fed rats. Kefir treatment also reduced the mRNA levels of key lipogenic genes sterol regulatory element-binding protein (SREBP-1c) and fatty acid synthase (FASN) together with a decline in expression of tumor necrosis factor-alpha (TNF-alpha), NF-kappa B, and glycosylated glycoprotein (CD68) in the liver. Moreover, kefir treatment improved insulin signaling at the level of insulin receptor substrate 1 (IRS-1) and phospho-endothelial nitric oxide synthase (peNOS) as well as fructose transporters (GLUT2 and GLUT5) in the liver, but not in the adipose tissue, of high-fructose-fed rats. Consequently, kefir supplementation suppresses hepatic lipogenesis and inflammatory status, but promotes insulin signaling, in association with a change of the fecal microbiota and attenuation of the intestinal permeability factors in high-fructose-fed rats. Thus, we propose that kefir has favorable effects on the hepatic and intestinal irregularities induced by fructose overconsumption.
dc.description.sponsorshipGazi University Research Fund [02/201814]
dc.description.sponsorshipThis study was supported by grants from the Gazi University Research Fund under Grant [02/201814] . The authors thank Dr. Hamdi Barbaros Odzer for his contributions to the preparation of the kefir.
dc.identifier.doi10.1016/j.foodres.2021.110287
dc.identifier.issn0963-9969
dc.identifier.issn1873-7145
dc.identifier.pmid33992387
dc.identifier.scopus2-s2.0-85102967946
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.foodres.2021.110287
dc.identifier.urihttps://hdl.handle.net/20.500.12587/25290
dc.identifier.volume143
dc.identifier.wosWOS:000663304300001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofFood Research International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_20241229
dc.subjectDietary fructose; Kefir; Microbiota; Permeability factors; Lipogenesis; Insulin signaling
dc.titlePotential mechanistic pathways underlying intestinal and hepatic effects of kefir in high-fructose-fed rats
dc.typeArticle

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