Preliminary characterization of biosurfactants produced by microorganisms isolated from refinery wastewaters

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dc.contributor.authorYalcin, Emine
dc.contributor.authorErgene, Aysun
dc.date.accessioned2020-06-25T17:51:22Z
dc.date.available2020-06-25T17:51:22Z
dc.date.issued2010
dc.departmentKırıkkale Üniversitesi
dc.description.abstractSome bacterial strains isolated from refinery wastewaters were identified as Pseudomonas aeruginosa RWI, Pseudomonas putida RWII, Pseudomonas fluorescens RWIII and Burkholderia cepacia RWIV, and the biosurfactants produced by these strains were coded as BS-I, BS-II, BS-III and BS-IV, respectively. The bacterial strains were characterized by the following biochemical methods: Gram stain, oxidase activity, indol, lactose and growth at 42 degrees C. Biosurfactant production was evaluated by: emulsification activity, surface tension measurement and critical micelle concentration. Chemical characterization of the biosurfactants was done by: FTIR and analysis of carbohydrate, protein and lipid content. The biosurfactants showed good emulsification activity against different hydrocarbon sources. The initial surface tension of culture broth was determined as 67.3 mN/m, and production of BS-I, BS-II, BS-III and BS-IV lowered this value to 35.9, 49.2, 51.6 and 45.7 mN/m, respectively. The critical micelle concentration of the biosurfactants was found to be in the range 10-50 mg/L. From the results of this study it was observed that the refinery wastewaters are a suitable source for isolation of biosurfactant-producing bacteria, but are not a substrate for biosurfactant production.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1080/09593330903426695
dc.identifier.endpage232en_US
dc.identifier.issn0959-3330
dc.identifier.issn1479-487X
dc.identifier.issue2en_US
dc.identifier.pmid20391807
dc.identifier.scopus2-s2.0-77649280112
dc.identifier.scopusqualityQ1
dc.identifier.startpage225en_US
dc.identifier.urihttps://doi.org/10.1080/09593330903426695
dc.identifier.urihttps://hdl.handle.net/20.500.12587/4828
dc.identifier.volume31en_US
dc.identifier.wosWOS:000275282800012
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofEnvironmental Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectrefinery wastewateren_US
dc.subjectbiosurfactanten_US
dc.subjectemulsion indexen_US
dc.subjectsurface tensionen_US
dc.subjectFTIRen_US
dc.titlePreliminary characterization of biosurfactants produced by microorganisms isolated from refinery wastewatersen_US
dc.typeArticle

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