Nephroprotective potential of carnitine against glycerol and contrast-induced kidney injury in rats through modulation of oxidative stress, proinflammatory cytokines, and apoptosis

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dc.contributor.authorKunak, Celalettin S.
dc.contributor.authorUgan, Rustem A.
dc.contributor.authorCadirci, Elif
dc.contributor.authorKarakus, Emre
dc.contributor.authorPolat, Beyzagul
dc.contributor.authorUn, Harun
dc.contributor.authorKaraman, Adem
dc.date.accessioned2020-06-25T18:22:31Z
dc.date.available2020-06-25T18:22:31Z
dc.date.issued2016
dc.departmentKırıkkale Üniversitesi
dc.descriptionKaraman, Adem/0000-0002-3091-0609; UGAN, RUSTEM ANIL/0000-0002-4837-2343; POLAT, Beyzagul/0000-0003-2042-5949; ATMACA, HASAN TARIK/0000-0001-8379-4114; UN, Harun/0000-0003-1772-282X
dc.description.abstractObjective: Contrast media (CM) are a major cause of nephropathy in high-risk patients. The aim of this study was to examine the effects of carnitine (CAR) in advanced nephrotoxicity due to CM administration in rats with glycerol-induced renal functional disorder. Methods: 40 rats were divided randomly into five groups (n=8): (1) healthy group; (2) glycerol only (GLY); (3) glycerol and CM (GLY1CM); (4) glycerol, CM and 200 mg kg(-1) carnitine (CAR200, Carnitene (R); Sigma-tau/Santa Farma, Istanbul, Turkey); and (5) glycerol, CM and 400 mg kg(-1) carnitine (CAR400). Kidney injury was induced with a single-dose, intramuscular injection of 10 ml kg(-1) body weight (b.w.) of GLY. CAR was administered intraperitoneally. CM (8 ml kg(-1) b.w. iohexol, Omni-paque (TM) T; Opakim Medical Products, Istanbul, Turkey) was infused via the tail vein to the rats in Groups 3-5. Results: L-carnitine administration significantly decreased serum creatinine and blood urea nitrogen levels. Superoxide dismutase and glutathione activity increased significantly in the treatment groups compared with the nephrotoxic groups. CAR400 significantly reduced malondialdehyde levels to healthy levels. In the treatment groups, tumour necrosis factor (TNF)-beta, transforming growth factor 1 beta, interleukin 1 beta and caspase-3 gene expression decreased compared with the nephrotoxic groups. TNF-alpha and nuclear factor kappa-beta (NF-kappa B) protein expression increased after CM and CAR administration reduced both TNF-alpha and NF-kappa B expressions. Histopathologically, hyaline and haemorrhagic casts and necrosis in proximal tubules increased in the nephrotoxicity groups and decreased in the CAR groups. Conclusion: The results reveal that L-carnitine protects the oxidant/antioxidant balance and decreases proinflammatory cytokines and apoptosis in CM-induced nephrotoxicity in rats with underlying pathology. Advances in knowledge: Depending on the underlying kidney pathologies, the incidence of CM-induced nephropathy (CIN) increases. Therefore, this is the best model to represent clinically observed CIN. Advances in knowledge: Depending on the underlying kidney pathologies, the incidence of CM-induced nephropathy (CIN) increases. Therefore, this is the best model to represent clinically observed CIN.en_US
dc.description.sponsorshipAtaturk University Scientific Experimental Project OfficeAtaturk University [2013/52]en_US
dc.description.sponsorshipThis work was supported by the Ataturk University Scientific Experimental Project Office through project number: 2013/52.en_US
dc.identifier.citationKunak CS, Ugan RA, Cadirci E, Karakus E, Polat B, Un H, et al. Nephroprotective potential of carnitine against glycerol and contrast-induced kidney injury in rats through modulation of oxidative stress, proinflammatory cytokines, and apoptosis. Br J Radiol 2016; 89: 20140724.en_US
dc.identifier.doi10.1259/bjr.20140724
dc.identifier.issn0007-1285
dc.identifier.issn1748-880X
dc.identifier.issue1058en_US
dc.identifier.pmid26562095
dc.identifier.scopus2-s2.0-84957895100
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1259/bjr.20140724
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6777
dc.identifier.volume89en_US
dc.identifier.wosWOS:000368422100001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBritish Inst Radiologyen_US
dc.relation.ispartofBritish Journal Of Radiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleNephroprotective potential of carnitine against glycerol and contrast-induced kidney injury in rats through modulation of oxidative stress, proinflammatory cytokines, and apoptosisen_US
dc.typeArticle

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