One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)

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dc.contributor.authorCoskun-Ari, Fatma Filiz
dc.contributor.authorGuldemir, Dilek
dc.contributor.authorDurmaz, Riza
dc.date.accessioned2020-06-25T17:52:22Z
dc.date.available2020-06-25T17:52:22Z
dc.date.issued2012
dc.departmentKırıkkale Üniversitesi
dc.descriptionari, filiz/0000-0002-8107-732X
dc.description.abstractThe life-threatening illnesses caused by Streptococcus pneumoniae have been declined significantly after the use of pneumococcal conjugate vaccines. Continuous monitoring of the vaccine serogroups/types is necessary to follow the changing epidemiology of invasive pneumococcal diseases. Recently, the sequential multiplex PCR approach, which uses several different sets of reactions, has been commonly adopted for determining capsular serogroups/types of S. pneumoniae isolates. In our study, we focused on development of a one-step multiplex PCR assay detecting all 1, 3, 4, 5, 6A/B, 7F, 9V, 14, 18C, 19A, 19F and 23F serogroups/types targeted by PCV13. The content of multiplex PCR mix and the cycling conditions were optimized in a manner that allowed rapid and accurate serotyping of a pneumococcal isolate by performing only a single amplification reaction. In our study of 182 clinical isolates, the one-step multiplex PCR assay exhibited 100% sensitivity and specificity, suggesting that its utilization can significantly reduce the use of traditional antiserum method requiring expensive reagents. Citation: Coskun-Ari FF, Guldemir D, Durmaz R (2012) One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13). PLoS ONE 7(12): e50406. doi:10.1371/journal.pone.0050406en_US
dc.identifier.citationCoskun-Ari F.F., Guldemir D., Durmaz R. (2012) One-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13- Valent Pneumococcal Conjugate Vaccine (PCV13). PLoS ONE 7(12): e50406en_US
dc.identifier.doi10.1371/journal.pone.0050406
dc.identifier.issn1932-6203
dc.identifier.issue12en_US
dc.identifier.pmid23226519
dc.identifier.scopus2-s2.0-84870741316
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1371/journal.pone.0050406
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5143
dc.identifier.volume7en_US
dc.identifier.wosWOS:000312108500016
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherPublic Library Scienceen_US
dc.relation.ispartofPlos One
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.titleOne-Step Multiplex PCR Assay for Detecting Streptococcus pneumoniae Serogroups/Types Covered by 13-Valent Pneumococcal Conjugate Vaccine (PCV13)en_US
dc.typeArticle

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