Cibacron Blue F3GA and Cu(II) derived poly(2-hydroxyethylmethacrylate) membranes for lysozyme adsorption
Yükleniyor...
Tarih
1998
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Bv
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Lysozyme adsorption onto Cibacron Blue F3GA and Cu(II) derived poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes was investigated. Microporous poly(HEMA) membranes were prepared by photopolymerization of HEMA. The triazine dye, Cibacron Blue F3GA was attached covalently as a dye-ligand. These dye-membranes with a swelling ratio of 58% (w/w), and carrying different amounts of Cibacron Blue F3GA (between 0.35 and 1.07 mu mol cm(-2)) were used in the lysozyme adsorption studies. The effect of initial concentration and pH on the adsorption efficiency of dye-derived and metal-chelated membranes were studied in a batch reactor. The effect of Cu(II) incorporation on lysozyme adsorption was also studied. The non-specific adsorption of lysozyme on the poly(HEMA) membranes was 0.9 mu g cm(-2). Cibacron Blue F3GA attachment significantly increased the lysozyme adsorption up to 133.3 mu gcm(-2) Lysozyme adsorption capacity of the Cu(II) incorporated membranes (165.1 mu g cm(-2)) was greater than that of the Cibacron Blue F3GA-attached membranes. More than 85% of the adsorbed lysozyme was desorbed in 1 h in the desorption medium containing 0.5 M potassium thiocyanate (KSCN) at pH 8.0. (C) 1998 Elsevier Science B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
dye affinity membranes, lysozyme adsorption, poly(HEMA), Cibacron Blue F3GA, metal chelates
Kaynak
Colloids And Surfaces B-Biointerfaces
WoS Q Değeri
Q3
Scopus Q Değeri
Q1
Cilt
11
Sayı
3
Künye
closedAccess
