Cibacron Blue F3GA and Cu(II) derived poly(2-hydroxyethylmethacrylate) membranes for lysozyme adsorption

Basit Öğe Kaydı
dc.contributor.authorDenizli, A
dc.contributor.authorSenel, S
dc.contributor.authorArica, MY
dc.date.accessioned2020-06-25T17:34:36Z
dc.date.available2020-06-25T17:34:36Z
dc.date.issued1998
dc.departmentKırıkkale Üniversitesi
dc.description.abstractLysozyme adsorption onto Cibacron Blue F3GA and Cu(II) derived poly(2-hydroxyethyl methacrylate) [poly(HEMA)] membranes was investigated. Microporous poly(HEMA) membranes were prepared by photopolymerization of HEMA. The triazine dye, Cibacron Blue F3GA was attached covalently as a dye-ligand. These dye-membranes with a swelling ratio of 58% (w/w), and carrying different amounts of Cibacron Blue F3GA (between 0.35 and 1.07 mu mol cm(-2)) were used in the lysozyme adsorption studies. The effect of initial concentration and pH on the adsorption efficiency of dye-derived and metal-chelated membranes were studied in a batch reactor. The effect of Cu(II) incorporation on lysozyme adsorption was also studied. The non-specific adsorption of lysozyme on the poly(HEMA) membranes was 0.9 mu g cm(-2). Cibacron Blue F3GA attachment significantly increased the lysozyme adsorption up to 133.3 mu gcm(-2) Lysozyme adsorption capacity of the Cu(II) incorporated membranes (165.1 mu g cm(-2)) was greater than that of the Cibacron Blue F3GA-attached membranes. More than 85% of the adsorbed lysozyme was desorbed in 1 h in the desorption medium containing 0.5 M potassium thiocyanate (KSCN) at pH 8.0. (C) 1998 Elsevier Science B.V. All rights reserved.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0927-7765(98)00027-7
dc.identifier.endpage122en_US
dc.identifier.issn0927-7765
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0032126187
dc.identifier.scopusqualityQ1
dc.identifier.startpage113en_US
dc.identifier.urihttps://doi.org/10.1016/S0927-7765(98)00027-7
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2799
dc.identifier.volume11en_US
dc.identifier.wosWOS:000075540900001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofColloids And Surfaces B-Biointerfaces
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectdye affinity membranesen_US
dc.subjectlysozyme adsorptionen_US
dc.subjectpoly(HEMA)en_US
dc.subjectCibacron Blue F3GAen_US
dc.subjectmetal chelatesen_US
dc.titleCibacron Blue F3GA and Cu(II) derived poly(2-hydroxyethylmethacrylate) membranes for lysozyme adsorptionen_US
dc.typeArticle

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