Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen

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dc.contributor.authorSahin, Aydan Acar
dc.contributor.authorAslim, Belma
dc.contributor.authorTan, Sema
dc.contributor.authorAlan, Senol
dc.contributor.authorPinar, Nur Munevver
dc.date.accessioned2020-06-25T18:29:32Z
dc.date.available2020-06-25T18:29:32Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.descriptionPinar, Nur Munevver/0000-0001-5466-795X; SAHIN, AYDAN ACAR/0000-0002-5350-5534
dc.description.abstractObjective: Cupressaceae pollen has commonly been reported to be an important aeroallergen and causal factor of spring, autumn and winter pollinosis in many countries. The aim of this study was to compare of the structure and allergenic protein content of Cupressus arizonica Greene., Cupressus sempervirens L. and Juniperus oxycedrus L. pollen in detail and contribute to Cupressaceae pollen allergen diagnosis and therapy studies in Turkey. Methods: The pollen structure were examined by LM and SEM. Pollen protein content was investigated by Bradford protein assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), Western blot analysis and two-dimensional polyacrylamide gel electrophoresis (2DE PAGE), respectively. Pectate lyase (PL) enzyme activities were compared. Immunoblotting was carried out by using extracts of the three taxa pollen collected from Turkey. Results: All three taxa was found very similar in terms of pollen morphology however, intine thickness was prominently different. Cupressus arizonica pollen extracts showed the lowest PL activity. Five sera specific IgE of all allergic subjects showed reaction with only C. arizonica pollen extracts. Conclusions: As a conclusion, the pollen structure, protein function or protein structure and isoforms of allergens could affects allergenic properties of the pollen. This study also may help to improve the Cupressaceae pollen allergen diagnosis and therapy.en_US
dc.description.sponsorshipBAP Research Council in Kirikkale University [2013/26]en_US
dc.description.sponsorshipThis study was funded BAP Research Council (Project no: 2013/26) in Kirikkale University. The author thanks Zeynep Misirligil for technical assistance and the Department of Immunology and Allergy Diseases, Ankara University School of Medicine, for giving access to patient sera and data. The authors also thanks to TUBITAK MAM Genetic Engineering and Biotechnology Institute, Kocaeli, Turkey for providing antibodies.en_US
dc.identifier.citationAcar Şahin A, Aslım B, Tan S, Alan Ş, Pınar N (2018). Differences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollen. Türk Biyokimya Dergisi, 43(4), 435 - 446.en_US
dc.identifier.doi10.1515/tjb-2017-0260
dc.identifier.endpage446en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue4en_US
dc.identifier.scopus2-s2.0-85040973644
dc.identifier.scopusqualityQ3
dc.identifier.startpage435en_US
dc.identifier.trdizinid309406
dc.identifier.urihttps://doi.org/10.1515/tjb-2017-0260
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7357
dc.identifier.volume43en_US
dc.identifier.wosWOS:000439144300010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal Of Biochemistry-Turk Biyokimya Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAllergyen_US
dc.subjectCupressaceaeen_US
dc.subjectPollenen_US
dc.subjectIsoformen_US
dc.subjectPectate lyaseen_US
dc.subjectTurkeyen_US
dc.titleDifferences in structure, allergenic protein content and pectate lyase enzyme activity of some Cupressaceae pollenen_US
dc.typeArticle

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