Optimisation of Indirect ELISA by Comparison of Different Antigen Preparations for Detection of Antibodies Against Schmallenberg Virus

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dc.contributor.authorAzkur, Ahmet Kursat
dc.contributor.authorAksoy, Emel
dc.contributor.authorYildirim, Murat
dc.contributor.authorYildiz, Kader
dc.date.accessioned2021-01-14T18:10:21Z
dc.date.available2021-01-14T18:10:21Z
dc.date.issued2020
dc.departmentKKÜ
dc.description.abstractSchmallenberg virus (SBV) infection, discovered in 2011, was reported in Europe including Turkey, Africa and recently in some Asian countries. Commercial enzyme-linked immunosorbent assay (ELISA) kits were widely used by researchers in many epidemiological studies and SBV diagnosis. The aim of this study was to optimise indirect in-house ELISA that is based on different antigen preparations of cell-culture derived whole SBV particle. Antigen preparations were maintained with various methods: PEG precipitation, ultracentrifugation, dialysis, and antigen inactivation. Following antigen optimisation, steps of antigen coating, blocking, conjugate and stop solution were optimised and in-house ELISA was compared to commercial indirect SBV ELISA kit. The best result in ELISA antigen preparation for SBV was gained by 30% PEG purification method followed by formaldehyde inactivation. Although results of this study demonstrated that in-house ELISA for detection of SBV specific antibodies was equally sensitive and specific as commercial kit, purified SBV antigen based in-house ELISA development could increase S/P ratios.en_US
dc.identifier.citationAzkur AK, Aksoy E, Yildirim M, Yildiz K: Optimisation of indirect ELISA by comparison of different antigen preparations for detection of antibodies against Schmallenberg virus. Kafkas Univ Vet Fak Derg, 26 (6): 795-800, 2020.en_US
dc.identifier.doi10.9775/kvfd.2020.24397
dc.identifier.endpage800en_US
dc.identifier.issn1300-6045
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85093107536
dc.identifier.scopusqualityQ3
dc.identifier.startpage795en_US
dc.identifier.trdizinid420201
dc.identifier.urihttps://doi.org/10.9775/kvfd.2020.24397
dc.identifier.urihttps://hdl.handle.net/20.500.12587/12538
dc.identifier.volume26en_US
dc.identifier.wosWOS:000582334700010
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherKAFKAS UNIV, VETERINER FAKULTESI DERGISIen_US
dc.relation.ispartofKAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDiagnosisen_US
dc.subjectDialysisen_US
dc.subjectELISAen_US
dc.subjectPEGen_US
dc.subjectSchmallenberg virusen_US
dc.titleOptimisation of Indirect ELISA by Comparison of Different Antigen Preparations for Detection of Antibodies Against Schmallenberg Virusen_US
dc.typeArticle

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