Dye-ligand and metal chelate poly(2-hydroxyethylmethacrylate) membranes for affinity separation of proteins

Basit Öğe Kaydı
dc.contributor.authorArica, MY
dc.contributor.authorTestereci, HN
dc.contributor.authorDenizli, A
dc.date.accessioned2020-06-25T17:34:37Z
dc.date.available2020-06-25T17:34:37Z
dc.date.issued1998
dc.departmentKırıkkale Üniversitesi
dc.description.abstractCibacron Blue F3GA was covalently immobilized onto poly(2-hydroxyethyl methacrylate) (pHEMA) membranes via the nucleophilic reaction between the chloride of its triazine ring and the hydroxyl group of pHEMA. Then, Fe3+ ions were complexed by chelation with the immobilized Cibacron Blue F3GA molecules. Different amounts of Fe3+ ions were loaded on the membranes by changing the concentration of Fe3+ ions and pH of the reaction medium. Membranes with or without Fe3+ were used in the adsorption of glucose oxidase, catalase and bovine serum albumin. The adsorption capacities of these membranes were determined by changing pH and the concentration of the proteins in the adsorption medium. The adsorption phenomena appeared to follow a typical Langmuir isotherm. The maximum capacities (q(m)) of the Fe3+ complexed membranes for glucose oxidase, catalase and bovine serum albumin (8.70.10(-3) mu mol m(-2), 2.15.10(-3) mu mol m(-3) and 2.21.10(-3) mu mol m(-2)) were greater than those of the untreated membranes (6.79.10(-3) mu mol m(-2), 1.34.10(-3) mu mol m(-2) and 1.94.10(-3) mu mol m(-2)) respectively. The nonspecific adsorption of the enzymes and the protein on the pHEMA membranes was negligible. (C) 1998 Elsevier Science B.V.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1016/S0021-9673(97)01079-0
dc.identifier.endpage91en_US
dc.identifier.issn0021-9673
dc.identifier.issn1873-3778
dc.identifier.issue1-2en_US
dc.identifier.pmid9550101
dc.identifier.scopus2-s2.0-0032513270
dc.identifier.scopusqualityQ1
dc.identifier.startpage83en_US
dc.identifier.urihttps://doi.org/10.1016/S0021-9673(97)01079-0
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2804
dc.identifier.volume799en_US
dc.identifier.wosWOS:000072721600008
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal Of Chromatography A
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectaffinity membranesen_US
dc.subjectmembranesen_US
dc.subjectmetal chelate affinity chromatographyen_US
dc.subjectpoly(2-hydroxyethyl methacrylate) membranesen_US
dc.subjectaffinity adsorbentsen_US
dc.subjectproteinsen_US
dc.subjectenzymesen_US
dc.subjectmetal chelatesen_US
dc.subjectironen_US
dc.subjectcibacron blue F3GAen_US
dc.titleDye-ligand and metal chelate poly(2-hydroxyethylmethacrylate) membranes for affinity separation of proteinsen_US
dc.typeArticle

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