Quantitative analysis of colonization with real-time PCR to identify the role of Oxalobacter formigenes in calcium oxalate urolithiasis

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dc.contributor.authorBatislam, Ertan
dc.contributor.authorYilmaz, Erdal
dc.contributor.authorYuvanc, Ercan
dc.contributor.authorKisa, Ozgul
dc.contributor.authorKisa, Ucler
dc.date.accessioned2020-06-25T17:52:36Z
dc.date.available2020-06-25T17:52:36Z
dc.date.issued2012
dc.departmentKırıkkale Üniversitesi
dc.descriptionKISA, Ucler/0000-0002-8131-6810; Batislam, Ertan/0000-0002-7493-4573
dc.description.abstractThe objective of the study was to quantitatively measure the number of Oxalobacter formigenes (O. formigenes) colonizations in the gastrointestinal tract in calcium oxalate-forming patients with real-time polymerase chain reaction (PCR). Calcium oxalate-forming patients (n: 27) were included in the study. Serum calcium, sodium, potassium, urea and creatinine levels, as well as 24 h urine levels of calcium and oxalate were measured. The numbers of O. formigenes colonies in stool samples were detected by real-time PCR. One or two metabolic abnormalities were detected in 15 of 27 patients. The O. formigenes levels in patients with metabolic disturbance were significantly decreased when compared to the patients with no metabolic abnormalities (p: 0.038). The undetectable levels of O. formigenes were encountered in one of five patients with hypercalciuria, in three of four patients with hyperoxaluria and in four of six patients with both hypercalciuria and hyperoxaluria. In nine patients with a history of stone recurrence, O. formigenes colonization was significantly lower than the patients with the first stone attack (p: 0.001). O. formigenes formation ceased or significantly diminished in patients with calcium oxalate stones with a coexistence of both hyperoxaluria and hypercalciuria. The measurement of O. formigenes colonies by real-time PCR seemed to be an inconvenient and expensive method. For this reason, the real-time PCR measurements can be spared for the patients with stone recurrences and with metabolic abnormalities like hypercalciuria and hyperoxaluria. The exact measurement of O. formigenes may also help more accurate programming of O. formigenes-based treatments.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1007/s00240-011-0449-8
dc.identifier.endpage460en_US
dc.identifier.issn0300-5623
dc.identifier.issue5en_US
dc.identifier.pmid22215293
dc.identifier.scopus2-s2.0-84867900560
dc.identifier.scopusqualityN/A
dc.identifier.startpage455en_US
dc.identifier.urihttps://doi.org/10.1007/s00240-011-0449-8
dc.identifier.urihttps://hdl.handle.net/20.500.12587/5187
dc.identifier.volume40en_US
dc.identifier.wosWOS:000308813800003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.ispartofUrological Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCalciumen_US
dc.subjectOxalateen_US
dc.subjectUrolithiasisen_US
dc.subjectOxalobacter formigenesen_US
dc.subjectReal-time PCRen_US
dc.titleQuantitative analysis of colonization with real-time PCR to identify the role of Oxalobacter formigenes in calcium oxalate urolithiasisen_US
dc.typeArticle

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