A research on Babesia and Theileria species in sheep and goats of Kirikkale province through molecular methods

Basit Öğe Kaydı
dc.authoridKaya, Ufuk/0000-0002-4805-0993
dc.contributor.authorGökpınar, Sami
dc.contributor.authorGazyagci, Aycan Nuriye
dc.contributor.authorAydenizöz, Meral
dc.contributor.authorKaya, Ufuk
dc.date.accessioned2025-01-21T16:35:23Z
dc.date.available2025-01-21T16:35:23Z
dc.date.issued2021
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe aim of the present study was to determine presence and prevalence of Babesia and Theileria species in the sheep and goats of stock farming in the province of Kirikkale, which is in Central Anatolia Region of Turkey through microscopic examination and RLB method. Venous blood samples of 3 mL were collected from vena jugularis of 300 sheep and 100 goats into tubes containing EDTA. Blood smears were prepared from samples; and stained by Giemsa and examined for Theileria and Babesia under a light microscope. Genomic DNAs were extracted from blood samples with EDTA through suitable methods; a region with a length of 360 to 430 bp on variable region V4 of the 18 ssu rRNA gene of Theileria and Babesia species were proliferated; PCR products obtained were hybridized on a membrane where species-specific probes. Piroplasms were detected in 54 (18%) sheep and in none of the goats during microscopic examination of blood smears. With the RLB technique; T. ovis 70%, Theileria sp. 2.3%, B. ovis 5.3%, T. ovis + B. ovis 2.7% were detected in sheep. In goats; T. ovis was detected at a rate of 1%. Consequently, this study is the first for detection of Theileria and Babesia species in sheep and goats in Kirikkale. Presence of T. ovis and B. ovis in the sheep, and T. ovis in goats was detected in Kirikkale.
dc.description.sponsorshipKirikkale University Scientific Research Coordination Unit [2014/119]
dc.description.sponsorshipThis research was supported by the Kirikkale University Scientific Research Coordination Unit (Project number: 2014/119).
dc.identifier.doi10.3906/vet-2103-17
dc.identifier.endpage919
dc.identifier.issn1300-0128
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85118794737
dc.identifier.scopusqualityQ3
dc.identifier.startpage912
dc.identifier.urihttps://doi.org/10.3906/vet-2103-17
dc.identifier.urihttps://hdl.handle.net/20.500.12587/24106
dc.identifier.volume45
dc.identifier.wosWOS:000711273500014
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTubitak Scientific & Technological Research Council Turkey
dc.relation.ispartofTurkish Journal of Veterinary & Animal Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241229
dc.subjectBabesia; goat; RLB; sheep; Theileria
dc.titleA research on Babesia and Theileria species in sheep and goats of Kirikkale province through molecular methods
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
Tam Metin/Full Text
Boyut:
437.54 KB
Biçim:
Adobe Portable Document Format
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu
TR-Dizin İndeksli Yayınlar Koleksiyonu

Kişisel bilgilerinizi şu amaçlarla toplar ve işleriz: Kimlik Doğrulama, Tercihler, Onay ve İstatistikler.
Daha fazla bilgi edinmek için lütfen gizlilik politikası sayfamızı okuyun.

Özelleştirme