Co-culture of rat luteal cells with islet cells enhances islet viability and revascularization

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dc.contributor.authorBoyuk, Gulbahar
dc.contributor.authorYigit, A. Arzu
dc.contributor.authorAydogan, Ilkay
dc.date.accessioned2020-06-25T18:29:22Z
dc.date.available2020-06-25T18:29:22Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.description/0000-0002-3453-2967; YIgit, Ayse Arzu/0000-0001-5837-6877
dc.description.abstractIslet cell transplantation is a major treatment strategy for type I diabetes, and has proven to be effective for maintaining glucose homeostasis. However, this treatment requires an extended period of immunosuppression to prevent rejection and recurrent transplantation to maintain function. Thus, to enhance the properties of transplanted islet cells, we examined the effect of the co-culture of luteal cells, which secrete progesterone, on islet cell viability, functionality, and revascularization. It was found that islet viability and functionality were higher in the co-cultured group than in single cultures of islets at 48 and 96 h, in parallel with increased progesterone and vascular endothelial growth factor (VEGF) secretion from luteal cells. In the co-culture groups, VEGF levels at 48 and 96 h and CD31 levels at 48 h were significantly higher than those in the islet groups (p < 0.001 and p < 0.05, respectively), and basic fibroblast growth factor (bFGF) levels were increased at 96 h (p < 0.001). Thus, co-culture with luteal cells may increase islet vascularity by enhancing VEGF and bFGF levels for up to 96 h, which could help to markedly increase the pre-transplantation time to allow for effective immunosuppression therapy. This method may also promote islet cell viability and functionality. Progesterone and angiogenic factors secreted from luteal cells may be responsible for these positive effects.en_US
dc.description.sponsorshipKU SRPCU [2015/128]; Turkish National Science Foundation (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [2214A]en_US
dc.description.sponsorshipThis study was supported by the KU SRPCU (2015/128). Additionally, we would like to thank the Turkish National Science Foundation (TUBITAK) for providing support for the study (scholarship 2214A).en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.1007/s11626-018-0286-y
dc.identifier.endpage647en_US
dc.identifier.issn1071-2690
dc.identifier.issn1543-706X
dc.identifier.issue9en_US
dc.identifier.pmid30187177
dc.identifier.scopus2-s2.0-85053392353
dc.identifier.scopusqualityQ3
dc.identifier.startpage640en_US
dc.identifier.urihttps://doi.org/10.1007/s11626-018-0286-y
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7280
dc.identifier.volume54en_US
dc.identifier.wosWOS:000446484500003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringeren_US
dc.relation.ispartofIn Vitro Cellular & Developmental Biology-Animal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectFunctionalityen_US
dc.subjectProgesteroneen_US
dc.subjectType I diabetesen_US
dc.subjectVascular endothelial growth factoren_US
dc.subjectVascularizationen_US
dc.titleCo-culture of rat luteal cells with islet cells enhances islet viability and revascularizationen_US
dc.typeArticle

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