Detection of progressive and regressive phase and LINE-1 retrotransposon in transfected dogs with transmissible venereal tumor during chemotherapy

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dc.contributor.authorVural, Sevil Atalay
dc.contributor.authorHaziroglu, Rifki
dc.contributor.authorVural, Mehmet R.
dc.contributor.authorPolat, Ibrahim M.
dc.contributor.authorTunc, Arda S.
dc.date.accessioned2020-06-25T18:29:27Z
dc.date.available2020-06-25T18:29:27Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.description.abstractCanine transmissible venereal tumor (CTVT) is a tumor that commonly occurs in genital and extragenital sites of both genders. Long interspersed nuclear elements (LINE-1) retrotransposon has a pivotal role in allogenic transfection among uncontrolled dog populations. This study aimed to perform pathomorphological, immunohistochemical, and in situ polymerase chain reaction (PCR) evaluation of CTVT (n = 18) in transfected dogs during chemotherapy. Immunohistochemically, tumor phases were investigated by using specific markers (CD3, CD4, CD8, CD79, and transforming growth factor beta [TGF-beta]), and investigated an amplified specific sequence of TVT LINE-1 retrotransposon by in situ PCR. Polyhedral-shaped neoplastic cells that had large, round, hypo/hyperchromatic nuclei and eosinophilic cytoplasm were detected. All marker results were positive, especially in the early weeks of recovery. CD4 and TGF-beta markers were conspicuously positive at the initial stage. In situ PCR LINE-1 sequence was initially positive in only four cases. It is believed that the CD and TGF-beta markers provide phase identification at tumor initiation and during chemotherapy. It is thought that presence of T and B lymphocytes, which have roles in cellular and humoral immunity, is needed so that regression of the tumor is possible.en_US
dc.description.sponsorshipAnkara University Scientific Research Projects, Turkey [10B3338008/2014]en_US
dc.description.sponsorshipThis study was supported by the Ankara University Scientific Research Projects (project No. 10B3338008/2014), Turkey.en_US
dc.identifier.citationAtalay Vural S, Haziroglu R, Vural MR, Polat IM, Tunc AS. Detection of progressive and regressive phase and LINE-1 retrotransposon in transfected dogs with transmissible venereal tumor during chemotherapy. J Vet Sci. 2018 Sep;19(5):620-626.en_US
dc.identifier.doi10.4142/jvs.2018.19.5.620
dc.identifier.endpage626en_US
dc.identifier.issn1229-845X
dc.identifier.issn1976-555X
dc.identifier.issue5en_US
dc.identifier.pmid30041285
dc.identifier.scopus2-s2.0-85054760220
dc.identifier.scopusqualityQ1
dc.identifier.startpage620en_US
dc.identifier.urihttps://doi.org/10.4142/jvs.2018.19.5.620
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7323
dc.identifier.volume19en_US
dc.identifier.wosWOS:000445944700005
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherKorean Soc Veterinary Scienceen_US
dc.relation.ispartofJournal Of Veterinary Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectantineoplastic agenten_US
dc.subjectimmunohistochemistryen_US
dc.subjectlong interspersed nuclear element-1en_US
dc.subjectvenereal tumorsen_US
dc.titleDetection of progressive and regressive phase and LINE-1 retrotransposon in transfected dogs with transmissible venereal tumor during chemotherapyen_US
dc.typeArticle

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