Comparison of Flow Cytometric Analysis and Eosin-Nigrosin Staining Methods for Determining some Morphological Characteristics of Bull Epididymal Spermatozoa

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dc.contributor.authorAkyol, Numan
dc.contributor.authorAzkur, Ahmet Kursat
dc.contributor.authorEkici, Husamettin
dc.date.accessioned2020-06-25T18:12:54Z
dc.date.available2020-06-25T18:12:54Z
dc.date.issued2015
dc.departmentKırıkkale Üniversitesi
dc.description.abstractThe aim of this study is to investigate necrosis and apoptosis in epididymal bull spermatozoa before freezing and after thawing using the flow cytometric method and to compare this with eosin-nigrosin dyeing, which is the conventional method used in assessing of spermatozoa. The testicles from fourteen bulls at local slaughterhouse were used for this study. The proportions of live spermatozoa, total apoptotic, necrotic and early necrotic spermatozoa levels were observed via flow cytometry. Annexin V/ PI fluoruscence dyeing was used to investigate the proprotions of apoptotic, necrotic, early necrotic and live spermatozoa for flow cytometry. The proportion of dead spermatozoa and protoplasmic droplets were determined using the eosin-nigrosin conventional dyeing method in fresh and frozen-thawed spermatozoa. The average dead spermatozoa count with flow cytometry was less than with the eosin-nigrosin method (P < 0.05). Some morphological characteristics such as protoplasmic droplets could be determined with the eosin-nigrosin method; however, sperm subpopulations entering the death process (apoptotic, necrotic and early necrotic) could be defined clearly only with the flow cytometric method. As a result, combination of eosin-nigrosin dyeing method and flow cytometric analysis of sperm morphological evaluation could give better results of bull epididymal semen in comparison to eosin-nigrosin dyeing method alone.en_US
dc.identifier.citationAkyol N., Azkur A. K., Ekici H. (2015). Comparison of Flow Cytometric Analysis and Eosin-Nigrosin Staining Methods for Determining some Morphological Characteristics of Bull Epididymal Spermatozoa. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(5), 659 - 664.en_US
dc.identifier.doi10.9775/kvfd.2015.13008
dc.identifier.endpage664en_US
dc.identifier.issn1300-6045
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-84934756214
dc.identifier.scopusqualityQ3
dc.identifier.startpage659en_US
dc.identifier.trdizinid174681
dc.identifier.urihttps://doi.org/10.9775/kvfd.2015.13008
dc.identifier.urihttps://hdl.handle.net/20.500.12587/6074
dc.identifier.volume21en_US
dc.identifier.wosWOS:000362666900006
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherKafkas Univ, Veteriner Fakultesi Dergisien_US
dc.relation.ispartofKafkas Universitesi Veteriner Fakultesi Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFlow cytometryen_US
dc.subjectEosin-nigrosinen_US
dc.subjectMorphological characteristicsen_US
dc.subjectEpidydimal spermatozoaen_US
dc.subjectBullen_US
dc.titleComparison of Flow Cytometric Analysis and Eosin-Nigrosin Staining Methods for Determining some Morphological Characteristics of Bull Epididymal Spermatozoaen_US
dc.typeArticle

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