The effects of topotecan on lipid peroxidation and antioxidant enzyme levels in rabbit liver tissue

Basit Öğe Kaydı
dc.contributor.authorKısa, Üçler
dc.contributor.authorÇağlayan, Osman
dc.contributor.authorKaçmaz, Murat
dc.date.accessioned2020-06-25T17:40:42Z
dc.date.available2020-06-25T17:40:42Z
dc.date.issued2005
dc.departmentKırıkkale Üniversitesi
dc.descriptionKISA, Ucler/0000-0002-8131-6810
dc.description.abstractBackground: To evaluate the possible influence of topotecan therapy on lipid peroxidation and antioxidant enzymes in rabbit liver tissue, the levels of thiobarbituric acid reactive substances (TBARS) and activities of the antioxidant enzymes superoxide dismutase ( SOD), glutathione peroxidase (GSH-Px), and catalase ( CAT) were investigated. Methods: A total of 24 adult, healthy New Zealand rabbits were divided into three groups (n = 8). Topotecan was administered intravenously via the ear vein for 3 days at 0.25 mg/kg/day for the low-dose group and 0.50 mg/kg/day for the high-dose group; physiological saline was used for the control group. All animals were sacrificed on day 15. Livers were removed and homogenised. The homogenate supernatant was used for measurement of TBARS levels, and SOD, GSH-Px, and CAT enzyme activities ( expressed as IU/mg protein). Results: There were significant differences in the TBARS level and GSH-Px activity between control and the topotecan treatment groups. TBARS level of topotecan treatment groups was higher than control (89% and 126%, respectively, P = 0.001). It was also significantly higher in the high-dose group than the low-dose group (20%; P = 0.011). GSH-Px activity was lower in the low- and high-dose topotecan groups than the control (42% [ P = 0.002] and 65% [ P = 0.001], respectively). Enzyme activity was lowest in the high-dose group and the difference was also significant when compared with the topotecan groups (40%; P = 0.001). Although there was some decrease in SOD and CAT activities in the topotecan-treated groups, differences from the control group were not significant. Conclusions: These findings suggest that topotecan treatment results in an increase in lipid peroxidation and a decrease in antioxidant enzyme activities in healthy liver tissue from rabbits. We believe that the addition of antioxidants to topotecan therapy may reduce the harmful effects of topotecan on liver tissue.en_US
dc.identifier.citationKisa, U., Caglayan, O., & Kacmaz, M. (2005). The effects of topotecan on lipid peroxidation and antioxidant enzyme levels in rabbit liver tissue. Redox Report, 10(2), 79–82. https://doi.org/10.1179/135100005X21705en_US
dc.identifier.doi10.1179/135100005X21705
dc.identifier.endpage82en_US
dc.identifier.issn1351-0002
dc.identifier.issn1743-2928
dc.identifier.issue2en_US
dc.identifier.pmid15949127
dc.identifier.scopus2-s2.0-33746375969
dc.identifier.scopusqualityQ2
dc.identifier.startpage79en_US
dc.identifier.urihttps://doi.org/10.1179/135100005X21705
dc.identifier.urihttps://hdl.handle.net/20.500.12587/3528
dc.identifier.volume10en_US
dc.identifier.wosWOS:000234388700003
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofRedox Report
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjecttopotecanen_US
dc.subjectlipid peroxidationen_US
dc.subjectantioxidant enzymesen_US
dc.titleThe effects of topotecan on lipid peroxidation and antioxidant enzyme levels in rabbit liver tissueen_US
dc.typeArticle

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
The effects of topotecan on lipid peroxidation and antioxidant enzyme levels in rabbit liver tissue.pdf
Boyut:
196 KB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin/Full Text
İndir

Koleksiyon

WOS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu