Neutrophil isolation from feline blood using discontinuous Percoll dilutions

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dc.contributor.authorSursal, Neslihan
dc.contributor.authorCakmak, Ayse
dc.contributor.authorYildiz, Kader
dc.date.accessioned2020-06-25T18:29:19Z
dc.date.available2020-06-25T18:29:19Z
dc.date.issued2018
dc.departmentKırıkkale Üniversitesi
dc.descriptionSursal, Neslihan/0000-0002-4144-9520; YILDIZ, Kader/0000-0001-5802-6156
dc.description.abstractObjective: Some studies have performed in vitro neutrophil isolation from feline blood. The major limiting factor for these studies is the small volume of blood that can be collected without development of potentially life-threatening complications. In the present study we attempted neutrophil isolation from feline venous blood samples using discontinuous Percoll gradients. Material and methods: Blood was collected from the cephalic vein of clinically healthy adult cats. The blood samples were layered on Percoll dilutions (72 %, 63%, 54% and 45%). After centrifugation, the feline polymorphonuclear leukocytes (PMN) accumulated as a band between 72-63% Percoll dilutions. The total cell count was calculated using light microscopy counts. The percentage of the neutrophils was determined microscopically after staining with Diff-Quik stain. Neutrophil viability was evaluated with a 0.01 % Trypan blue assay. The activation was determined based on intact cell morphology in the isolated neutrophils. Results: The mean PMN number was 22 x 10(5) per ml (minimum-maximum: 20-26 x 10(5)ml). Neutrophil homogeneity was > 95% in the cell suspensions. The viability of isolated neutrophils was > 98%. The technique did not result in neutrophil activation. Conclusion and clinical relevance: Discontinuous Percoll gradients (72%, 63 %, 54% and 45%) can be used to isolate neutrophils from blood samples of cats. The technique was simple to perform and neutrophil activation was minimal.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.doi10.15654/TPK-180577
dc.identifier.endpage402en_US
dc.identifier.issn1434-1239
dc.identifier.issn2567-5842
dc.identifier.issue6en_US
dc.identifier.pmid30658367
dc.identifier.scopus2-s2.0-85060127203
dc.identifier.scopusqualityQ3
dc.identifier.startpage399en_US
dc.identifier.urihttps://doi.org/10.15654/TPK-180577
dc.identifier.urihttps://hdl.handle.net/20.500.12587/7225
dc.identifier.volume46en_US
dc.identifier.wosWOS:000456471100006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherGeorg Thieme Verlag Kgen_US
dc.relation.ispartofTieraerztliche Praxis Ausgabe Kleintiere Heimtiere
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaten_US
dc.subjectPMNen_US
dc.subjectvenous blooden_US
dc.subjectisolationen_US
dc.subjectdiscontinuous Percoll gradientsen_US
dc.titleNeutrophil isolation from feline blood using discontinuous Percoll dilutionsen_US
dc.typeArticle

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