Immobilization of catalase in poly(isopropylacrylamide-co-hydroxyethylmethacrylate) thermally reversible hydrogels

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dc.contributor.authorArica, MY
dc.contributor.authorOktem, HA
dc.contributor.authorOktem, Z
dc.contributor.authorTuncel, SA
dc.date.accessioned2020-06-25T17:34:39Z
dc.date.available2020-06-25T17:34:39Z
dc.date.issued1999
dc.departmentKırıkkale Üniversitesi
dc.description.abstractCatalase was entrapped in thermally reversible poly (isopropylacrylamide-co-hydroxyethyl-methacrylate) (pNIPAM/HEMA) copolymer hydrogels. The thermoresponsive hydrogels, in cylindIl-rical geometry, were prepared in an aqueous buffer by redox polymerization. It was observed that upon entrapment, the activity retention of catalase was decreased between 47 and 14%, and that increasing the catalase loading of hydrogel adversely affected the activity. The kinetic behaviour of the entrapped enzyme was investigated in a batch reactor. The apparent kinetic constant of the entrapped enzyme was determined by the application of lMichaeiis-Menten model and indicated that the overall reaction rate was controlled by the substrate diffusion rate through the hydrogel matrix. Due to the thermoresponsive character of the hydrogel matrix, the maximum activity was achieved at 25 degrees C with the immobilized enzyme. The K-m value for immobilized catalase (28.6mM) was higher than that of free enzyme (16.5mM). Optimum pH was the same for both free and immobilized enzyme. Operational, thermal and storage stabilities of the enzyme were found to increase with immobilization. (C) 1999 Society of Chemical Industry.en_US
dc.identifier.citationclosedAccessen_US
dc.identifier.endpage884en_US
dc.identifier.issn0959-8103
dc.identifier.issue9en_US
dc.identifier.scopus2-s2.0-0033195456
dc.identifier.scopusqualityQ1
dc.identifier.startpage879en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12587/2820
dc.identifier.volume48en_US
dc.identifier.wosWOS:000082364300021
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherJohn Wiley & Sons Ltden_US
dc.relation.ispartofPolymer International
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectcatalaseen_US
dc.subjectthermo-responsive gelsen_US
dc.subjecthydrogelsen_US
dc.subjectentrapmenten_US
dc.subjectimmobilizationen_US
dc.titleImmobilization of catalase in poly(isopropylacrylamide-co-hydroxyethylmethacrylate) thermally reversible hydrogelsen_US
dc.typeArticle

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